Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site

Herbert Seiler, Verena Schmidt, Mareike Wenning, Siegfried Scherer

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26 Scopus citations

Abstract

Three Gram-staining-positive, strictly aerobic, motile, catalase-positive, endospore-forming rods, designated WCC 4582T, WCC 4581 and WCC 4583, were isolated from two different food sources and a pharmaceuticals production site. The three isolates were highly similar in their 16S rRNA gene sequences (100% similarity) and groEL sequences (99.2-100% similarity), Fouriertransform infrared spectroscopic fingerprints and other features tested. The isolates were most closely related to Bacillus horneckiae; the isolates and the type strain of B. horneckiae shared 97.6 % and 89.6% 16S rRNA gene and groEL sequence similarities, respectively. The organisms grew optimally at 30 °C, at pH 7 and in the presence of 0.5% (w/v) NaCl. The cell-wall peptidoglycan of WCC 4582T contained meso-diaminopimelic acid (A1γ) and the genomic DNA G+C content was 36.4 mol%. DNA-DNA relatedness between strain WCC 4582T and B. horneckiae NRRL B-59162T was 17 %. The three isolates are considered to represent a novel species of the genus Bacillus, for which the name Bacillus kochii sp. nov. is proposed. The type strain is WCC 4582T (=DSM 23667T=CCUG 59877T=LMG 25855T).

Original languageEnglish
Pages (from-to)1092-1097
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume62
Issue number5
DOIs
StatePublished - May 2012

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