Bacillus gottheilii sp. nov., isolated from a pharmaceutical manufacturing site

A novel Gram-staining-positive, rod-shaped, motile, strictly aerobic, endospore-forming bacterium, designated WCC 4585 ^T, was isolated from a pharmaceutical production line. The organism grew optimally at 30 °C, at pH 8 and in the presence of 0.5 % (w/v) NaCl. Oval endospores were formed subterminally and terminally in swollen sporangia. The cell-wall diamino acid was meso-diaminopimelic acid (type A1γ) and the genomic DNA G+C content was 38.7 mol%. The major menaquinone was MK-7. The cellular fatty acid profile contained major amounts of iso-C _{15: 0}, anteiso-C _{15: 0} and anteiso-C _{17: 0}, and the cellular phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. The isolate was most closely related to Bacillus oceanisediminis H2 ^T, Bacillus infantis SMC 4352-1 ^T, Bacillus firmus NCIMB 9366 ^T, Bacillus circulans ATCC 4513 ^T and Bacillus horneckiae DSM 23495 ^T with which it shared less than 98.0 % 16S rRNA gene sequence similarity. DNA-DNA relatedness values between strain WCC 4585 ^T and five type strains of related species were ≤27 % and sequence similarity values based on groEL sequences were ≤88.7 %. On the basis of the characteristics presented, strain WCC 4585T is proposed to represent a novel species, Bacillus gottheilii sp. nov. The type strain is WCC 4585 ^T(= DSM 23668 ^T= CCUG 59876 ^T= LMG 25856 ^T).