Axon branch removal at developing synapses by axosome shedding

Derron L. Bishop, Thomas Misgeld, Mark K. Walsh, Wen Biao Gan, Jeff W. Lichtman

Research output: Contribution to journalArticlepeer-review

227 Scopus citations


In many parts of the developing nervous system, the number of axonal inputs to each postsynaptic cell is dramatically reduced. This synapse elimination has been extensively studied at the neuromuscular junction, but how axons are lost is unknown. Here, we combine time-lapse imaging of fluorescently labeled axons and serial electron microscopy to show that axons at neuromuscular junctions are removed by an unusual cellular mechanism. As axons disappear, they shed numerous membrane bound remnants. These "axosomes" contain a high density of synaptic organelles and are formed by engulfment of axon tips by Schwann cells. After this engulfment, the axosome's contents mix with the cytoplasm of the glial cell. Axosome shedding might underlie other forms of axon loss and may provide a pathway for interactions between axons and glia.

Original languageEnglish
Pages (from-to)651-661
Number of pages11
Issue number4
StatePublished - 18 Nov 2004
Externally publishedYes


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