Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity

Julius Nückel; Elisa Planatscher; Anne Wiebe Mohr; Karolin Deichl; Hrvoje Mijočević; Martin Feuerherd; Lisa Wolff; Johanna Erber; Jochen Schneider; Michael Quante; Christoph Winter; Jürgen Ruland; Alexander Hapfelmeier; Wolfgang Hammerschmidt; Andreas Moosmann; Ulrike Protzer; Uta Behrends; Josef Mautner

doi:10.3389/fimmu.2022.889836

Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity

Julius Nückel, Elisa Planatscher, Anne Wiebe Mohr, Karolin Deichl, Hrvoje Mijočević, Martin Feuerherd, Lisa Wolff, Johanna Erber, Jochen Schneider, Michael Quante, Christoph Winter, Jürgen Ruland, Alexander Hapfelmeier, Wolfgang Hammerschmidt, Andreas Moosmann, Ulrike Protzer, Uta Behrends, Josef Mautner

Default

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Understanding immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial to contain the COVID-19 pandemic. Using a multiplex approach, serum IgG responses against the whole SARS-CoV-2 proteome and the nucleocapsid proteins of endemic human coronaviruses (HCoVs) were measured in SARS-CoV-2-infected donors and healthy controls. COVID-19 severity strongly correlated with IgG responses against the nucleocapsid (N) of SARS-CoV-2 and possibly with the number of viral antigens targeted. Furthermore, a strong correlation between COVID-19 severity and serum responses against N of endemic alpha- but not betacoronaviruses was detected. This correlation was neither caused by cross-reactivity of antibodies, nor by a general boosting effect of SARS-CoV-2 infection on pre-existing humoral immunity. These findings raise the prospect of a potential disease progression marker for COVID-19 severity that allows for early stratification of infected individuals.

Original language	English
Article number	889836
Journal	Frontiers in Immunology
Volume	13
DOIs	https://doi.org/10.3389/fimmu.2022.889836
State	Published - 7 Sep 2022

Keywords

COVID-19
SARS-CoV-2
coronavirus
multiplex serology
nucleocapsid protein

Access to Document

10.3389/fimmu.2022.889836

Cite this

Nückel, J., Planatscher, E., Mohr, A. W., Deichl, K., Mijočević, H., Feuerherd, M., Wolff, L., Erber, J., Schneider, J., Quante, M., Winter, C., Ruland, J., Hapfelmeier, A., Hammerschmidt, W., Moosmann, A., Protzer, U., Behrends, U., & Mautner, J. (2022). Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity. Frontiers in Immunology, 13, Article 889836. https://doi.org/10.3389/fimmu.2022.889836

@article{027fdb45bbea49e294f1b437ae28f303,

title = "Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity",

abstract = "Understanding immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial to contain the COVID-19 pandemic. Using a multiplex approach, serum IgG responses against the whole SARS-CoV-2 proteome and the nucleocapsid proteins of endemic human coronaviruses (HCoVs) were measured in SARS-CoV-2-infected donors and healthy controls. COVID-19 severity strongly correlated with IgG responses against the nucleocapsid (N) of SARS-CoV-2 and possibly with the number of viral antigens targeted. Furthermore, a strong correlation between COVID-19 severity and serum responses against N of endemic alpha- but not betacoronaviruses was detected. This correlation was neither caused by cross-reactivity of antibodies, nor by a general boosting effect of SARS-CoV-2 infection on pre-existing humoral immunity. These findings raise the prospect of a potential disease progression marker for COVID-19 severity that allows for early stratification of infected individuals.",

keywords = "COVID-19, SARS-CoV-2, coronavirus, multiplex serology, nucleocapsid protein",

author = "Julius N{\"u}ckel and Elisa Planatscher and Mohr, {Anne Wiebe} and Karolin Deichl and Hrvoje Mijo{\v c}evi{\'c} and Martin Feuerherd and Lisa Wolff and Johanna Erber and Jochen Schneider and Michael Quante and Christoph Winter and J{\"u}rgen Ruland and Alexander Hapfelmeier and Wolfgang Hammerschmidt and Andreas Moosmann and Ulrike Protzer and Uta Behrends and Josef Mautner",

note = "Publisher Copyright: Copyright {\textcopyright} 2022 N{\"u}ckel, Planatscher, Mohr, Deichl, Mijo{\v c}evi{\'c}, Feuerherd, Wolff, Erber, Schneider, Quante, Winter, Ruland, Hapfelmeier, Hammerschmidt, Moosmann, Protzer, Behrends and Mautner.",

year = "2022",

month = sep,

day = "7",

doi = "10.3389/fimmu.2022.889836",

language = "English",

volume = "13",

journal = "Frontiers in Immunology",

issn = "1664-3224",

publisher = "Frontiers Media S.A.",

}

Nückel, J, Planatscher, E, Mohr, AW, Deichl, K, Mijočević, H, Feuerherd, M, Wolff, L, Erber, J, Schneider, J, Quante, M, Winter, C, Ruland, J, Hapfelmeier, A, Hammerschmidt, W, Moosmann, A, Protzer, U, Behrends, U & Mautner, J 2022, 'Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity', Frontiers in Immunology, vol. 13, 889836. https://doi.org/10.3389/fimmu.2022.889836

TY - JOUR

T1 - Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity

AU - Nückel, Julius

AU - Planatscher, Elisa

AU - Mohr, Anne Wiebe

AU - Deichl, Karolin

AU - Mijočević, Hrvoje

AU - Feuerherd, Martin

AU - Wolff, Lisa

AU - Erber, Johanna

AU - Schneider, Jochen

AU - Quante, Michael

AU - Winter, Christoph

AU - Ruland, Jürgen

AU - Hapfelmeier, Alexander

AU - Hammerschmidt, Wolfgang

AU - Moosmann, Andreas

AU - Protzer, Ulrike

AU - Behrends, Uta

AU - Mautner, Josef

N1 - Publisher Copyright: Copyright © 2022 Nückel, Planatscher, Mohr, Deichl, Mijočević, Feuerherd, Wolff, Erber, Schneider, Quante, Winter, Ruland, Hapfelmeier, Hammerschmidt, Moosmann, Protzer, Behrends and Mautner.

PY - 2022/9/7

Y1 - 2022/9/7

N2 - Understanding immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial to contain the COVID-19 pandemic. Using a multiplex approach, serum IgG responses against the whole SARS-CoV-2 proteome and the nucleocapsid proteins of endemic human coronaviruses (HCoVs) were measured in SARS-CoV-2-infected donors and healthy controls. COVID-19 severity strongly correlated with IgG responses against the nucleocapsid (N) of SARS-CoV-2 and possibly with the number of viral antigens targeted. Furthermore, a strong correlation between COVID-19 severity and serum responses against N of endemic alpha- but not betacoronaviruses was detected. This correlation was neither caused by cross-reactivity of antibodies, nor by a general boosting effect of SARS-CoV-2 infection on pre-existing humoral immunity. These findings raise the prospect of a potential disease progression marker for COVID-19 severity that allows for early stratification of infected individuals.

AB - Understanding immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial to contain the COVID-19 pandemic. Using a multiplex approach, serum IgG responses against the whole SARS-CoV-2 proteome and the nucleocapsid proteins of endemic human coronaviruses (HCoVs) were measured in SARS-CoV-2-infected donors and healthy controls. COVID-19 severity strongly correlated with IgG responses against the nucleocapsid (N) of SARS-CoV-2 and possibly with the number of viral antigens targeted. Furthermore, a strong correlation between COVID-19 severity and serum responses against N of endemic alpha- but not betacoronaviruses was detected. This correlation was neither caused by cross-reactivity of antibodies, nor by a general boosting effect of SARS-CoV-2 infection on pre-existing humoral immunity. These findings raise the prospect of a potential disease progression marker for COVID-19 severity that allows for early stratification of infected individuals.

KW - COVID-19

KW - SARS-CoV-2

KW - coronavirus

KW - multiplex serology

KW - nucleocapsid protein

UR - http://www.scopus.com/inward/record.url?scp=85138385078&partnerID=8YFLogxK

U2 - 10.3389/fimmu.2022.889836

DO - 10.3389/fimmu.2022.889836

M3 - Article

C2 - 36159804

AN - SCOPUS:85138385078

SN - 1664-3224

VL - 13

JO - Frontiers in Immunology

JF - Frontiers in Immunology

M1 - 889836

ER -

Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this