Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting

Nis Borbye-Lorenzen; Yacila I. Deza-Lougovski; Solveig Holmgaard; Luzia M. Weiss; Marie Bækvad-Hansen; Kristin Skogstrand; Anna Rieckmann; Axel Börsch-Supan; Martina Börsch-Supan

doi:10.1002/dad2.12555

Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting

Nis Borbye-Lorenzen, Yacila I. Deza-Lougovski, Solveig Holmgaard, Luzia M. Weiss, Marie Bækvad-Hansen, Kristin Skogstrand, Anna Rieckmann, Axel Börsch-Supan, Martina Börsch-Supan

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

INTRODUCTION: The apolipoprotein E (APOE) ε4 allele is associated with high risk for Alzheimer's disease. It is unclear whether individual levels of the circulating apoE4 protein in ε4 carriers confer additional risk. Measuring apoE4 protein levels from dried blood spots (DBS) has the potential to provide information on genetic status as well as circulating levels and to include these measures in large survey settings. METHODS: We developed a multiplex immunoassay to detect apoE4 protein levels in DBS from 15,974 participants, aged 50+ from Wave 6 of the Survey of Health, Ageing and Retirement in Europe (SHARE). RESULTS: The apoE4 protein signal was presented in two separable distributions. One distribution corresponded to carriers of at least one copy of the ε4 allele. Fieldwork cofounders affected protein levels but did not explain individual differences. DISCUSSION: Future research should investigate how genotype and apoE4 level interact with lifestyle and other variables to impact cognitive aging.

Original language	English
Article number	e12555
Journal	Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring
Volume	16
Issue number	1
DOIs	https://doi.org/10.1002/dad2.12555
State	Published - 1 Jan 2024
Externally published	Yes

Keywords

Ageing and Retirement in Europe
Alzheimer's disease
Survey of Health
apolipoprotein E ε4
dried blood spots
survey research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/dad2.12555

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Borbye-Lorenzen, N., Deza-Lougovski, Y. I., Holmgaard, S., Weiss, L. M., Bækvad-Hansen, M., Skogstrand, K., Rieckmann, A., Börsch-Supan, A., & Börsch-Supan, M. (2024). Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting. Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring, 16(1), Article e12555. https://doi.org/10.1002/dad2.12555

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title = "Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting",

abstract = "INTRODUCTION: The apolipoprotein E (APOE) ε4 allele is associated with high risk for Alzheimer's disease. It is unclear whether individual levels of the circulating apoE4 protein in ε4 carriers confer additional risk. Measuring apoE4 protein levels from dried blood spots (DBS) has the potential to provide information on genetic status as well as circulating levels and to include these measures in large survey settings. METHODS: We developed a multiplex immunoassay to detect apoE4 protein levels in DBS from 15,974 participants, aged 50+ from Wave 6 of the Survey of Health, Ageing and Retirement in Europe (SHARE). RESULTS: The apoE4 protein signal was presented in two separable distributions. One distribution corresponded to carriers of at least one copy of the ε4 allele. Fieldwork cofounders affected protein levels but did not explain individual differences. DISCUSSION: Future research should investigate how genotype and apoE4 level interact with lifestyle and other variables to impact cognitive aging.",

keywords = "Ageing and Retirement in Europe, Alzheimer's disease, Survey of Health, apolipoprotein E ε4, dried blood spots, survey research",

author = "Nis Borbye-Lorenzen and Deza-Lougovski, {Yacila I.} and Solveig Holmgaard and Weiss, {Luzia M.} and Marie B{\ae}kvad-Hansen and Kristin Skogstrand and Anna Rieckmann and Axel B{\"o}rsch-Supan and Martina B{\"o}rsch-Supan",

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Borbye-Lorenzen, N, Deza-Lougovski, YI, Holmgaard, S, Weiss, LM, Bækvad-Hansen, M, Skogstrand, K, Rieckmann, A, Börsch-Supan, A & Börsch-Supan, M 2024, 'Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting', Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring, vol. 16, no. 1, e12555. https://doi.org/10.1002/dad2.12555

TY - JOUR

T1 - Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting

AU - Borbye-Lorenzen, Nis

AU - Deza-Lougovski, Yacila I.

AU - Holmgaard, Solveig

AU - Weiss, Luzia M.

AU - Bækvad-Hansen, Marie

AU - Skogstrand, Kristin

AU - Rieckmann, Anna

AU - Börsch-Supan, Axel

AU - Börsch-Supan, Martina

PY - 2024/1/1

Y1 - 2024/1/1

N2 - INTRODUCTION: The apolipoprotein E (APOE) ε4 allele is associated with high risk for Alzheimer's disease. It is unclear whether individual levels of the circulating apoE4 protein in ε4 carriers confer additional risk. Measuring apoE4 protein levels from dried blood spots (DBS) has the potential to provide information on genetic status as well as circulating levels and to include these measures in large survey settings. METHODS: We developed a multiplex immunoassay to detect apoE4 protein levels in DBS from 15,974 participants, aged 50+ from Wave 6 of the Survey of Health, Ageing and Retirement in Europe (SHARE). RESULTS: The apoE4 protein signal was presented in two separable distributions. One distribution corresponded to carriers of at least one copy of the ε4 allele. Fieldwork cofounders affected protein levels but did not explain individual differences. DISCUSSION: Future research should investigate how genotype and apoE4 level interact with lifestyle and other variables to impact cognitive aging.

AB - INTRODUCTION: The apolipoprotein E (APOE) ε4 allele is associated with high risk for Alzheimer's disease. It is unclear whether individual levels of the circulating apoE4 protein in ε4 carriers confer additional risk. Measuring apoE4 protein levels from dried blood spots (DBS) has the potential to provide information on genetic status as well as circulating levels and to include these measures in large survey settings. METHODS: We developed a multiplex immunoassay to detect apoE4 protein levels in DBS from 15,974 participants, aged 50+ from Wave 6 of the Survey of Health, Ageing and Retirement in Europe (SHARE). RESULTS: The apoE4 protein signal was presented in two separable distributions. One distribution corresponded to carriers of at least one copy of the ε4 allele. Fieldwork cofounders affected protein levels but did not explain individual differences. DISCUSSION: Future research should investigate how genotype and apoE4 level interact with lifestyle and other variables to impact cognitive aging.

KW - Ageing and Retirement in Europe

KW - Alzheimer's disease

KW - Survey of Health

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JO - Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring

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IS - 1

M1 - e12555

ER -

Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting

Abstract

Keywords

UN SDGs

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