TY - JOUR
T1 - Assessment of a pro-healing stent in an animal model of early neoatherosclerosis
AU - Nicol, Philipp
AU - Lutter, Christoph
AU - Bulin, Anna
AU - Castellanos, Maria Isabel
AU - Lenz, Tobias
AU - Hoppmann, Petra
AU - Lahmann, Anna Lena
AU - Colleran, Roisin
AU - Euller, Kristina
AU - Steigerwald, Kristin
AU - Neubauer, Stefanie
AU - Rechenmacher, Florian
AU - Ludwig, Beatrice Stefanie
AU - Weinmüller, Michael
AU - Kerch, Garry
AU - Guo, Liang
AU - Cheng, Qi
AU - Acampado, Eduardo
AU - Koppara, Tobias
AU - Kessler, Horst
AU - Joner, Michael
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Background: Neoatherosclerosis represents an accelerated manifestation of atherosclerosis in nascent neointima after stenting, associated with adverse events. We investigated whether improved reendothelialization using RGD-coated stents results in diminished vascular permeability and reduced foam cell formation compared to standard DES in atherosclerotic rabbits. Methods and Results: Neointimal foam cell formation was induced in rabbits (n = 7). Enhanced endothelial integrity in RGD-coated stents resulted in decreased vascular permeability relative to DES, which was further confirmed by SEM and TEM. Cell culture experiments examined the effect of everolimus on endothelial integrity. Increasing concentrations of everolimus resulted in a dose-dependent decrease of endothelial cell junctions and foam cell transformation of monocytes, confirming the relevance of endothelial integrity in preventing permeability of LDL. Conclusion: Incomplete endothelial integrity was confirmed as a key factor of neointimal foam cell formation following stent implantation. Pro-healing stent coatings may facilitate reendothelialization and reduce the risk of neoatherosclerosis.
AB - Background: Neoatherosclerosis represents an accelerated manifestation of atherosclerosis in nascent neointima after stenting, associated with adverse events. We investigated whether improved reendothelialization using RGD-coated stents results in diminished vascular permeability and reduced foam cell formation compared to standard DES in atherosclerotic rabbits. Methods and Results: Neointimal foam cell formation was induced in rabbits (n = 7). Enhanced endothelial integrity in RGD-coated stents resulted in decreased vascular permeability relative to DES, which was further confirmed by SEM and TEM. Cell culture experiments examined the effect of everolimus on endothelial integrity. Increasing concentrations of everolimus resulted in a dose-dependent decrease of endothelial cell junctions and foam cell transformation of monocytes, confirming the relevance of endothelial integrity in preventing permeability of LDL. Conclusion: Incomplete endothelial integrity was confirmed as a key factor of neointimal foam cell formation following stent implantation. Pro-healing stent coatings may facilitate reendothelialization and reduce the risk of neoatherosclerosis.
UR - https://www.scopus.com/pages/publications/85084964634
U2 - 10.1038/s41598-020-64940-2
DO - 10.1038/s41598-020-64940-2
M3 - Article
C2 - 32427835
AN - SCOPUS:85084964634
SN - 2045-2322
VL - 10
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 8227
ER -