Assessing permeability alterations of the blood-bone marrow barrier due to total body irradiation: In vivo quantification with contrast enhanced magnetic resonance imaging

H. E. Daldrup-Link, T. M. Link, E. J. Rummeny, C. August, S. Könemann, H. Jürgens, W. Heindel

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Our aim was to quantify irradiation-induced permeability alterations of the blood-bone marrow barrier (BMB) with dynamic contrast enhanced magnetic resonance imaging (MRI). The standard small molecular contrast agent, gadoterate meglumine, and a new macromolecular contrast agent, carboxymethyldextran-Gd-DOTA (CMD-Gd-DOTA), were compared. Twenty New Zealand white rabbits underwent MRI of the bone marrow before and 1-2 days after total body irradiation (TBI). Dynamic, repetitive T1-weighted MRI was performed before and after injection of either 0.05 mmol/kg BW CMD-Gd-DOTA (n = 10) or 0.5 mmol/kg BW gadoterate (n = 10), Bone marrow contrast enhancement was quantified as delta signal intensity: ΔSI = (SI(post)- SI(pre)) / SI(pre *) 100%. All MRI data were compared with the histopathologic BMB ultrastructure. Dynamic bone marrow ΔSI data steadily increased after CMD-Gd-DOTA injection, while blood ΔSI data slightly decreased. This hone marrow contrast enhancement, indicative of contrast agent extravasation, was significantly higher and prolonged in the irradiated group as compared to non-irradiated controls (P < 0.05) and corresponded to irradiation-induced alterations of the BMB ultrastructure seen on electron microscopy. By contrast, ΔSI data of non-irradiated and irradiated marrow were not significantly different following gadoterate injection (P > 0.05). We conclude that irradiation-induced alterations in BMB permeability could be reliably assessed with dynamic MRI, using the new macromolecular contrast agent CMD-Gd-DOTA.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalBone Marrow Transplantation
Volume25
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Blood-bone marrow barrier
  • MRI
  • Macromolecular contrast agents

Fingerprint

Dive into the research topics of 'Assessing permeability alterations of the blood-bone marrow barrier due to total body irradiation: In vivo quantification with contrast enhanced magnetic resonance imaging'. Together they form a unique fingerprint.

Cite this