Assembly of a fluorescent cyclodextrin polyrotaxane and its detection by fluorescence microscopy

Gerhard Wenz; Iris Kräuter; Erich Sackmann

doi:10.1002/pola.23666

Assembly of a fluorescent cyclodextrin polyrotaxane and its detection by fluorescence microscopy

Gerhard Wenz, Iris Kräuter, Erich Sackmann

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Abstract

A straightforward method for the assembly of fluorescent polyrotaxanes is described here. The consecutive threading of N-(6I-desoxy-b-cyclodextrin-6I-yl)- N0-(5-fluoresceinyl)-thiourea, b-CD-F, and a-CD onto poly(N,N-dimethyliminium- hexamethylen-N0,N0-dimethyliminium-decamethylene chloride), I-6,10, leads to a fluorescent polyrotaxane. Free b-CD-F could be distinguished from threaded b-CD-F by gel electrophoresis. Since the dissociation of threaded a-CD rings is sterically hindered, the obtained polyrotaxanes are kinetically stable at 25 °C, and they did not require further stabilization by the attachment of stopper groups at the chain ends. Single polyrotaxane entities could be visualized with both fluorescence microscopy and atomic force microscopy.

Original language	English
Pages (from-to)	6223-6230
Number of pages	8
Journal	Journal of Polymer Science, Part A: Polymer Chemistry
Volume	47
Issue number	22
DOIs	https://doi.org/10.1002/pola.23666
State	Published - 15 Nov 2009

Keywords

Fluorescence
Molecular recognition
Polyamines
Polyrotaxanes

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10.1002/pola.23666

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title = "Assembly of a fluorescent cyclodextrin polyrotaxane and its detection by fluorescence microscopy",

abstract = "A straightforward method for the assembly of fluorescent polyrotaxanes is described here. The consecutive threading of N-(6I-desoxy-b-cyclodextrin-6I-yl)- N0-(5-fluoresceinyl)-thiourea, b-CD-F, and a-CD onto poly(N,N-dimethyliminium- hexamethylen-N0,N0-dimethyliminium-decamethylene chloride), I-6,10, leads to a fluorescent polyrotaxane. Free b-CD-F could be distinguished from threaded b-CD-F by gel electrophoresis. Since the dissociation of threaded a-CD rings is sterically hindered, the obtained polyrotaxanes are kinetically stable at 25 °C, and they did not require further stabilization by the attachment of stopper groups at the chain ends. Single polyrotaxane entities could be visualized with both fluorescence microscopy and atomic force microscopy.",

keywords = "Fluorescence, Molecular recognition, Polyamines, Polyrotaxanes",

author = "Gerhard Wenz and Iris Kr{\"a}uter and Erich Sackmann",

year = "2009",

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day = "15",

doi = "10.1002/pola.23666",

language = "English",

volume = "47",

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journal = "Journal of Polymer Science, Part A: Polymer Chemistry",

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T1 - Assembly of a fluorescent cyclodextrin polyrotaxane and its detection by fluorescence microscopy

AU - Wenz, Gerhard

AU - Kräuter, Iris

AU - Sackmann, Erich

PY - 2009/11/15

Y1 - 2009/11/15

N2 - A straightforward method for the assembly of fluorescent polyrotaxanes is described here. The consecutive threading of N-(6I-desoxy-b-cyclodextrin-6I-yl)- N0-(5-fluoresceinyl)-thiourea, b-CD-F, and a-CD onto poly(N,N-dimethyliminium- hexamethylen-N0,N0-dimethyliminium-decamethylene chloride), I-6,10, leads to a fluorescent polyrotaxane. Free b-CD-F could be distinguished from threaded b-CD-F by gel electrophoresis. Since the dissociation of threaded a-CD rings is sterically hindered, the obtained polyrotaxanes are kinetically stable at 25 °C, and they did not require further stabilization by the attachment of stopper groups at the chain ends. Single polyrotaxane entities could be visualized with both fluorescence microscopy and atomic force microscopy.

AB - A straightforward method for the assembly of fluorescent polyrotaxanes is described here. The consecutive threading of N-(6I-desoxy-b-cyclodextrin-6I-yl)- N0-(5-fluoresceinyl)-thiourea, b-CD-F, and a-CD onto poly(N,N-dimethyliminium- hexamethylen-N0,N0-dimethyliminium-decamethylene chloride), I-6,10, leads to a fluorescent polyrotaxane. Free b-CD-F could be distinguished from threaded b-CD-F by gel electrophoresis. Since the dissociation of threaded a-CD rings is sterically hindered, the obtained polyrotaxanes are kinetically stable at 25 °C, and they did not require further stabilization by the attachment of stopper groups at the chain ends. Single polyrotaxane entities could be visualized with both fluorescence microscopy and atomic force microscopy.

KW - Fluorescence

KW - Molecular recognition

KW - Polyamines

KW - Polyrotaxanes

UR - http://www.scopus.com/inward/record.url?scp=70350227178&partnerID=8YFLogxK

U2 - 10.1002/pola.23666

DO - 10.1002/pola.23666

M3 - Article

AN - SCOPUS:70350227178

SN - 0887-624X

VL - 47

SP - 6223

EP - 6230

JO - Journal of Polymer Science, Part A: Polymer Chemistry

JF - Journal of Polymer Science, Part A: Polymer Chemistry

IS - 22

ER -

Assembly of a fluorescent cyclodextrin polyrotaxane and its detection by fluorescence microscopy

Abstract

Keywords

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