Assays to characterize molecular chaperone function in vitro

Martin Haslbeck, Johannes Buchner

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Here, we describe a set of assays, using mitochondrial citrate synthase as a model substrate, which are suitable to test for chaperone function of proteins in vitro. Additionally, these assays distinguish between the ability of suppressing the aggregation of diverse substrate proteins by stable interaction (holdase function) and the ability to assist the refolding of substrate proteins (foldase function).

Original languageEnglish
Pages (from-to)39-51
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume1292
DOIs
StatePublished - 2015

Keywords

  • Aggregation suppression
  • Chaperone assay
  • Chaperone function
  • Citrate synthase
  • Protein aggregation
  • Protein folding
  • Protein refolding

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