Abstract
Here, we describe a set of assays, using mitochondrial citrate synthase as a model substrate, which are suitable to test for chaperone function of proteins in vitro. Additionally, these assays distinguish between the ability of suppressing the aggregation of diverse substrate proteins by stable interaction (holdase function) and the ability to assist the refolding of substrate proteins (foldase function).
Original language | English |
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Pages (from-to) | 39-51 |
Number of pages | 13 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1292 |
DOIs | |
State | Published - 2015 |
Keywords
- Aggregation suppression
- Chaperone assay
- Chaperone function
- Citrate synthase
- Protein aggregation
- Protein folding
- Protein refolding