Artificial maturation of [FeFe] hydrogenase in a redox polymer film

Christina Felbek; Steffen Hardt; Cecilia Papini; Debajyoti Pramanik; Vincent Artero; Marc Fontecave; Vincent Fourmond; Nicolas Plumeré; Christophe Léger

doi:10.1039/d0cc08168j

Artificial maturation of [FeFe] hydrogenase in a redox polymer film

Christina Felbek, Steffen Hardt, Cecilia Papini, Debajyoti Pramanik, Vincent Artero, Marc Fontecave, Vincent Fourmond, Nicolas Plumeré, Christophe Léger

Associate Professorship of Electrobiotechnology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We demonstrate that the insertion of the dinuclear active site of [FeFe] hydrogenase into the apo-enzyme can occur when the enzyme is embedded in a film of redox polymer, under conditions of mediated electron transfer. The maturation can be monitored by electrochemistry, and is as fast as under conditions of direct electron transfer. This new approach further clears the way to the implementation of hydrogenases in large scale real life processes.

Original language	English
Pages (from-to)	1750-1753
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	14
DOIs	https://doi.org/10.1039/d0cc08168j
State	Published - 18 Feb 2021

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abstract = "We demonstrate that the insertion of the dinuclear active site of [FeFe] hydrogenase into the apo-enzyme can occur when the enzyme is embedded in a film of redox polymer, under conditions of mediated electron transfer. The maturation can be monitored by electrochemistry, and is as fast as under conditions of direct electron transfer. This new approach further clears the way to the implementation of hydrogenases in large scale real life processes.",

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AU - Felbek, Christina

AU - Hardt, Steffen

AU - Papini, Cecilia

AU - Pramanik, Debajyoti

AU - Artero, Vincent

AU - Fontecave, Marc

AU - Fourmond, Vincent

AU - Plumeré, Nicolas

AU - Léger, Christophe

PY - 2021/2/18

Y1 - 2021/2/18

N2 - We demonstrate that the insertion of the dinuclear active site of [FeFe] hydrogenase into the apo-enzyme can occur when the enzyme is embedded in a film of redox polymer, under conditions of mediated electron transfer. The maturation can be monitored by electrochemistry, and is as fast as under conditions of direct electron transfer. This new approach further clears the way to the implementation of hydrogenases in large scale real life processes.

AB - We demonstrate that the insertion of the dinuclear active site of [FeFe] hydrogenase into the apo-enzyme can occur when the enzyme is embedded in a film of redox polymer, under conditions of mediated electron transfer. The maturation can be monitored by electrochemistry, and is as fast as under conditions of direct electron transfer. This new approach further clears the way to the implementation of hydrogenases in large scale real life processes.

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U2 - 10.1039/d0cc08168j

DO - 10.1039/d0cc08168j

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AN - SCOPUS:85100937013

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VL - 57

SP - 1750

EP - 1753

JO - Chemical Communications

JF - Chemical Communications

IS - 14

ER -

Artificial maturation of [FeFe] hydrogenase in a redox polymer film

Abstract

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