Artificial Gel-Based Organelles for Spatial Organization of Cell-Free Gene Expression Reactions

Lukas Aufinger, Friedrich C. Simmel

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Gel-based artificial organelles have been developed that enable sequence-specific and programmable localization of cell-free transcription and translation reactions inside an artificial cellular system. To this end, we utilize agarose microgels covalently modified with DNA templates coding for various functions and encapsulate them into emulsion droplets. We show that RNA signals transcribed from transcription organelles can be specifically targeted to capture organelles via hybridization to the corresponding DNA addresses. We also demonstrate that mRNA molecules, produced from transcription organelles and controlled by toehold switch riboregulators, are only translated in translation organelles containing their cognate DNA triggers. Spatial confinement of transcription and translation in separate organelles is thus superficially similar to gene expression in eukaryotic cells. Combining communicating gel spheres with specialized functions opens up new possibilities for programming artificial cellular systems at the organelle level.

Original languageEnglish
Pages (from-to)17245-17248
Number of pages4
JournalAngewandte Chemie International Edition in English
Volume57
Issue number52
DOIs
StatePublished - 21 Dec 2018

Keywords

  • artificial cells
  • cell-free gene expression
  • compartments
  • microgels
  • organelles

Fingerprint

Dive into the research topics of 'Artificial Gel-Based Organelles for Spatial Organization of Cell-Free Gene Expression Reactions'. Together they form a unique fingerprint.

Cite this