Artificial Cell on a Chip Integrated with Protein Degradation

Taishi Tonooka; Henrike Niederholtmeyer; Lev Tsimring; Jeff Hasty

doi:10.1109/MEMSYS.2019.8870666

Artificial Cell on a Chip Integrated with Protein Degradation

Taishi Tonooka
, Henrike Niederholtmeyer
, Lev Tsimring
, Jeff Hasty

Kyoto Institute of Technology
University of California, San Diego
University of California

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

This paper reports a microfluidic artificial cell system integrated with protein degradation. Previous artificial cells constructed in microfluidic devices only mimicked protein production. However, protein degradation is also important to reconstitute more complex cellular systems. Here, we realized, for the first time, artificial cells on a chip that can both produce and degrade proteins by adding a protein degradation system to the previous cell-free protein expression system in microchambers. Using the developed artificial cell on a chip, an oscillatory gene circuit requiring production and degradation of proteins was demonstrated.

Original language	English
Title of host publication	2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	107-109
Number of pages	3
ISBN (Electronic)	9781728116105
DOIs	https://doi.org/10.1109/MEMSYS.2019.8870666
State	Published - Jan 2019
Externally published	Yes
Event	32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 - Seoul, Korea, Republic of Duration: 27 Jan 2019 → 31 Jan 2019

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2019-January
ISSN (Print)	1084-6999

Conference

Conference	32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019
Country/Territory	Korea, Republic of
City	Seoul
Period	27/01/19 → 31/01/19

Access to Document

10.1109/MEMSYS.2019.8870666

Cite this

Tonooka, T., Niederholtmeyer, H., Tsimring, L., & Hasty, J. (2019). Artificial Cell on a Chip Integrated with Protein Degradation. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019 (pp. 107-109). Article 8870666 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2019.8870666

Tonooka, Taishi ; Niederholtmeyer, Henrike ; Tsimring, Lev et al. / Artificial Cell on a Chip Integrated with Protein Degradation. 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 107-109 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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title = "Artificial Cell on a Chip Integrated with Protein Degradation",

abstract = "This paper reports a microfluidic artificial cell system integrated with protein degradation. Previous artificial cells constructed in microfluidic devices only mimicked protein production. However, protein degradation is also important to reconstitute more complex cellular systems. Here, we realized, for the first time, artificial cells on a chip that can both produce and degrade proteins by adding a protein degradation system to the previous cell-free protein expression system in microchambers. Using the developed artificial cell on a chip, an oscillatory gene circuit requiring production and degradation of proteins was demonstrated.",

author = "Taishi Tonooka and Henrike Niederholtmeyer and Lev Tsimring and Jeff Hasty",

note = "Publisher Copyright: {\textcopyright} 2019 IEEE.; 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 ; Conference date: 27-01-2019 Through 31-01-2019",

year = "2019",

month = jan,

doi = "10.1109/MEMSYS.2019.8870666",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

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Tonooka, T, Niederholtmeyer, H, Tsimring, L & Hasty, J 2019, Artificial Cell on a Chip Integrated with Protein Degradation. in 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019., 8870666, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2019-January, Institute of Electrical and Electronics Engineers Inc., pp. 107-109, 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019, Seoul, Korea, Republic of, 27/01/19. https://doi.org/10.1109/MEMSYS.2019.8870666

Artificial Cell on a Chip Integrated with Protein Degradation. / Tonooka, Taishi; Niederholtmeyer, Henrike; Tsimring, Lev et al.
2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc., 2019. p. 107-109 8870666 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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N2 - This paper reports a microfluidic artificial cell system integrated with protein degradation. Previous artificial cells constructed in microfluidic devices only mimicked protein production. However, protein degradation is also important to reconstitute more complex cellular systems. Here, we realized, for the first time, artificial cells on a chip that can both produce and degrade proteins by adding a protein degradation system to the previous cell-free protein expression system in microchambers. Using the developed artificial cell on a chip, an oscillatory gene circuit requiring production and degradation of proteins was demonstrated.

AB - This paper reports a microfluidic artificial cell system integrated with protein degradation. Previous artificial cells constructed in microfluidic devices only mimicked protein production. However, protein degradation is also important to reconstitute more complex cellular systems. Here, we realized, for the first time, artificial cells on a chip that can both produce and degrade proteins by adding a protein degradation system to the previous cell-free protein expression system in microchambers. Using the developed artificial cell on a chip, an oscillatory gene circuit requiring production and degradation of proteins was demonstrated.

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M3 - Conference contribution

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Tonooka T, Niederholtmeyer H, Tsimring L, Hasty J. Artificial Cell on a Chip Integrated with Protein Degradation. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc. 2019. p. 107-109. 8870666. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2019.8870666

Artificial Cell on a Chip Integrated with Protein Degradation

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