Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3

Marie Kristin Nagel, Kamila Kalinowska, Karin Vogel, Gregory D. Reynolds, Zhixiang Wu, Franziska Anzenberger, Mie Ichikawa, Chie Tsutsumi, Masa H. Sato, Bernhard Kuster, Sebastian Y. Bednarek, Erika Isono

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Clathrin-mediated endocytosis of plasma membrane proteins is an essential regulatory process that controls plasma membrane protein abundance and is therefore important for many signaling pathways, such as hormone signaling and biotic and abiotic stress responses. On endosomal sorting, plasma membrane proteins maybe recycled or targeted for vacuolar degradation, which is dependent on ubiquitin modification of the cargos and is driven by the endosomal sorting complexes required for transport (ESCRTs). Components of the ESCRT machinery are highly conserved among eukaryotes, but homologs of ESCRT-0 that are responsible for recognition and concentration of ubiquitylated proteins are absent in plants. Recently several ubiquitin-binding proteins have been identified that serve in place of ESCRT-0; however, their function in ubiquitin recognition and endosomal trafficking is not well understood yet. In this study, we identified Src homology-3 (SH3) domain-containing protein 2 (SH3P2) as a ubiquitin- and ESCRT-I–binding protein that functions in intracellular trafficking. SH3P2 colocalized with clathrin light chain-labeled punctate structures and interacted with clathrin heavy chain in planta, indicating a role for SH3P2 in clathrin-mediated endocytosis. Furthermore, SH3P2 cofractionates with clathrin-coated vesicles (CCVs), suggesting that it associates with CCVs in planta. Mutants of SH3P2 and VPS23 genetically interact, suggesting that they could function in the same pathway. Based on these results, we suggest a role of SH3P2 as an ubiquitin-binding protein that binds and transfers ubiquitylated proteins to the ESCRT machinery.

Original languageEnglish
Pages (from-to)E7197-E7204
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number34
DOIs
StatePublished - 22 Aug 2017

Keywords

  • Arabidopsis
  • Clathrin
  • DUB
  • ESCRT
  • Ubiquitin

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