Arabidopsis D6PK is a lipid domain-dependent mediator of root epidermal planar polarity

Thomas Stanislas, Anke Hüser, Inês C.R. Barbosa, Christian S. Kiefer, Klaus Brackmann, Stefano Pietra, Anna Gustavsson, Melina Zourelidou, Claus Schwechheimer, Markus Grebe

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Development of diverse multicellular organisms relies on coordination of single-cell polarities within the plane of the tissue layer (planar polarity). Cell polarity often involves plasma membrane heterogeneity generated by accumulation of specific lipids and proteins into membrane subdomains. Coordinated hair positioning along Arabidopsis root epidermal cells provides a planar polarity model in plants, but knowledge about the functions of proteo-lipid domains in planar polarity signalling remains limited. Here we show that Rho-of-plant (ROP) 2 and 6, phosphatidylinositol-4-phosphate 5-kinase 3 (PIP5K3), DYNAMIN-RELATED PROTEIN (DRP) 1A and DRP2B accumulate in a sterol-enriched, polar membrane domain during root hair initiation. DRP1A, DRP2B, PIP5K3 and sterols are required for planar polarity and the AGCVIII kinase D6 PROTEIN KINASE (D6PK) is a modulator of this process. D6PK undergoes phosphatidylinositol-4,5-bisphosphate- and sterol-dependent basal-To-planar polarity switching into the polar, lipid-enriched domain just before hair formation, unravelling lipid-dependent D6PK localization during late planar polarity signalling.

Original languageEnglish
Article number15162
JournalNature Plants
Volume1
DOIs
StatePublished - 2 Nov 2015

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