Application of the incremental identification method to the formate oxidation using formate dehydrogenase

Claas Michalik, Thomas Schmidt, Michael Zavrel, Marion Ansorge-Schumacher, Antje Spiess, Wolfgang Marquardt

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

In this paper we apply the incremental method for the identification of reaction kinetics [Brendel, M., Bonvin, D., Marquardt, W., 2006. Incremental identification of complex reaction kinetics in homogeneous systems. Chemical Engineering Science 61, 5404-5420] to the oxidation of formate by dehydrogenase. For the identification task little a priori knowledge is assumed and thus a high number of model candidates have to be considered. In order to identify the best model from a set of candidates the number of candidates is efficiently reduced in a first step using the incremental method. In a second step the best model and its parameters are determined based on the reduced candidate set in a statistically sound manner using the classical simultaneous method.

Original languageEnglish
Pages (from-to)5592-5597
Number of pages6
JournalChemical Engineering Science
Volume62
Issue number18-20
DOIs
StatePublished - Sep 2007
Externally publishedYes

Keywords

  • Biocatalysis
  • Biochemical engineering
  • Enzyme
  • Formate dehydrogenase
  • Model identification
  • Parameter estimation
  • Reaction engineering

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