Application of ion-pair high-performance liquid chromatography to detection of the atypical coproporphyrin isomers II and IV in human faeces

K. Jacob; E. Egeler; B. Hennel; D. Neumeier; P. Luppa

doi:10.1016/0378-4347(91)80497-Z

Application of ion-pair high-performance liquid chromatography to detection of the atypical coproporphyrin isomers II and IV in human faeces

K. Jacob, E. Egeler, B. Hennel, D. Neumeier, P. Luppa

Ludwig-Maximilians-Universität München

Research output: Contribution to journal › Article › peer-review

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Abstract

A highly selective and sensitive method has been developed for the detection of small amounts of the atypical isomers II and IV of coproporphyrin in human faeces. This method combines liquid-liquid extraction and solid-phase sampling techniques using talc and C₁₈-modified silica gel as the sorbents. Simultaneous separation of the four coproporphyrin isomers I-IV was achieved by isocratic ion-pair high-performance liquid chromatography. Stool samples of healthy subjects (n = 12) contained 1.1 ± 0.4% (mean ± S.D.) isomer II and 2.2 ± 0.9% isomer IV of total coproporphyrins. A somewhat higher content of isomer II (2.7%) and isomer IV (5.4%) was found in faeces of a patient suffering from porphyria variegata.

Original language	English
Pages (from-to)	317-320
Number of pages	4
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	572
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-4347(91)80497-Z
State	Published - 6 Dec 1991
Externally published	Yes

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@article{35b3c9bc610a46cfae10f8dda0381385,

title = "Application of ion-pair high-performance liquid chromatography to detection of the atypical coproporphyrin isomers II and IV in human faeces",

abstract = "A highly selective and sensitive method has been developed for the detection of small amounts of the atypical isomers II and IV of coproporphyrin in human faeces. This method combines liquid-liquid extraction and solid-phase sampling techniques using talc and C18-modified silica gel as the sorbents. Simultaneous separation of the four coproporphyrin isomers I-IV was achieved by isocratic ion-pair high-performance liquid chromatography. Stool samples of healthy subjects (n = 12) contained 1.1 ± 0.4% (mean ± S.D.) isomer II and 2.2 ± 0.9% isomer IV of total coproporphyrins. A somewhat higher content of isomer II (2.7%) and isomer IV (5.4%) was found in faeces of a patient suffering from porphyria variegata.",

author = "K. Jacob and E. Egeler and B. Hennel and D. Neumeier and P. Luppa",

note = "Funding Information: The continuous financial support of our work by the Hans-Fischer-Gesellschaft (Munich, Germany) is gratefully acknowledged.",

year = "1991",

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doi = "10.1016/0378-4347(91)80497-Z",

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T1 - Application of ion-pair high-performance liquid chromatography to detection of the atypical coproporphyrin isomers II and IV in human faeces

AU - Jacob, K.

AU - Egeler, E.

AU - Hennel, B.

AU - Neumeier, D.

AU - Luppa, P.

N1 - Funding Information: The continuous financial support of our work by the Hans-Fischer-Gesellschaft (Munich, Germany) is gratefully acknowledged.

PY - 1991/12/6

Y1 - 1991/12/6

N2 - A highly selective and sensitive method has been developed for the detection of small amounts of the atypical isomers II and IV of coproporphyrin in human faeces. This method combines liquid-liquid extraction and solid-phase sampling techniques using talc and C18-modified silica gel as the sorbents. Simultaneous separation of the four coproporphyrin isomers I-IV was achieved by isocratic ion-pair high-performance liquid chromatography. Stool samples of healthy subjects (n = 12) contained 1.1 ± 0.4% (mean ± S.D.) isomer II and 2.2 ± 0.9% isomer IV of total coproporphyrins. A somewhat higher content of isomer II (2.7%) and isomer IV (5.4%) was found in faeces of a patient suffering from porphyria variegata.

AB - A highly selective and sensitive method has been developed for the detection of small amounts of the atypical isomers II and IV of coproporphyrin in human faeces. This method combines liquid-liquid extraction and solid-phase sampling techniques using talc and C18-modified silica gel as the sorbents. Simultaneous separation of the four coproporphyrin isomers I-IV was achieved by isocratic ion-pair high-performance liquid chromatography. Stool samples of healthy subjects (n = 12) contained 1.1 ± 0.4% (mean ± S.D.) isomer II and 2.2 ± 0.9% isomer IV of total coproporphyrins. A somewhat higher content of isomer II (2.7%) and isomer IV (5.4%) was found in faeces of a patient suffering from porphyria variegata.

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U2 - 10.1016/0378-4347(91)80497-Z

DO - 10.1016/0378-4347(91)80497-Z

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AN - SCOPUS:0026351545

SN - 0378-4347

VL - 572

SP - 317

EP - 320

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

IS - 1-2

ER -

Application of ion-pair high-performance liquid chromatography to detection of the atypical coproporphyrin isomers II and IV in human faeces

Abstract

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