TY - JOUR
T1 - Application of fluorescence dye saturation labeling for differential proteome analysis of 1,000 microdissected cells from pancreatic ductal adenocarcinoma precursor lesions.
AU - Sitek, Barbara
AU - Sipos, Bence
AU - Klöppel, Günter
AU - Schmiegel, Wolff
AU - Hahn, Stephan A.
AU - Meyer, Helmut E.
AU - Stühler, Kai
PY - 2008
Y1 - 2008
N2 - The identification of molecular changes underlying clinical pathogenic processes is often hampered by significant cellular diversity of the tissue. Pathogenic aberrant cells are surrounded by cells originating e.g., from stroma, the vascular system or other neighbouring cell types, which lead to under-representation of interesting cells when analysing whole tissue specimen. Therefore, selection of relevant cell types for detailed analysis is an absolute prerequisite for in depth elucidation of underlying biological processes. Microdissection offers the advantage to select for a biologically relevant cell type which is often in low abundance. Here, we present a proteomics approach allowing us to analyse 1,000 microdissected cells stemming from pancreatic carcinoma precursor lesions applying fluorescence dye saturation labeling.
AB - The identification of molecular changes underlying clinical pathogenic processes is often hampered by significant cellular diversity of the tissue. Pathogenic aberrant cells are surrounded by cells originating e.g., from stroma, the vascular system or other neighbouring cell types, which lead to under-representation of interesting cells when analysing whole tissue specimen. Therefore, selection of relevant cell types for detailed analysis is an absolute prerequisite for in depth elucidation of underlying biological processes. Microdissection offers the advantage to select for a biologically relevant cell type which is often in low abundance. Here, we present a proteomics approach allowing us to analyse 1,000 microdissected cells stemming from pancreatic carcinoma precursor lesions applying fluorescence dye saturation labeling.
UR - http://www.scopus.com/inward/record.url?scp=44449136554&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-210-0_1
DO - 10.1007/978-1-60327-210-0_1
M3 - Article
C2 - 18369882
AN - SCOPUS:44449136554
SN - 1064-3745
VL - 425
SP - 1
EP - 14
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -