Application of 235 rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control

Edith Frahm; Ines Heiber; Sandra Hoffmann; Cornelia Koob; Harald Meier; Wolfgang Ludwig; Rudolf Amann; Karl H. Schleifer; Ursula Obst

doi:10.1016/S0723-2020(98)80055-6

Application of 235 rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control

Edith Frahm, Ines Heiber, Sandra Hoffmann, Cornelia Koob, Harald Meier, Wolfgang Ludwig, Rudolf Amann, Karl H. Schleifer, Ursula Obst

TUM Emeriti of Excellence

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.

Original language	English
Pages (from-to)	450-453
Number of pages	4
Journal	Systematic and Applied Microbiology
Volume	21
Issue number	3
DOIs	https://doi.org/10.1016/S0723-2020(98)80055-6
State	Published - Aug 1998

Keywords

23S rDNA-targeted oligonucleotide probes
Enterococci
Hybridization
Water hygiene

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10.1016/S0723-2020(98)80055-6

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title = "Application of 235 rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control",

abstract = "Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.",

keywords = "23S rDNA-targeted oligonucleotide probes, Enterococci, Hybridization, Water hygiene",

author = "Edith Frahm and Ines Heiber and Sandra Hoffmann and Cornelia Koob and Harald Meier and Wolfgang Ludwig and Rudolf Amann and Schleifer, {Karl H.} and Ursula Obst",

year = "1998",

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T1 - Application of 235 rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control

AU - Frahm, Edith

AU - Heiber, Ines

AU - Hoffmann, Sandra

AU - Koob, Cornelia

AU - Meier, Harald

AU - Ludwig, Wolfgang

AU - Amann, Rudolf

AU - Schleifer, Karl H.

AU - Obst, Ursula

PY - 1998/8

Y1 - 1998/8

N2 - Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.

AB - Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.

KW - 23S rDNA-targeted oligonucleotide probes

KW - Enterococci

KW - Hybridization

KW - Water hygiene

UR - http://www.scopus.com/inward/record.url?scp=0031595294&partnerID=8YFLogxK

U2 - 10.1016/S0723-2020(98)80055-6

DO - 10.1016/S0723-2020(98)80055-6

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SN - 0723-2020

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EP - 453

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 3

ER -

Application of 235 rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control

Abstract

Keywords

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