Application of 235 rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control

Edith Frahm, Ines Heiber, Sandra Hoffmann, Cornelia Koob, Harald Meier, Wolfgang Ludwig, Rudolf Amann, Karl H. Schleifer, Ursula Obst

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.

Original languageEnglish
Pages (from-to)450-453
Number of pages4
JournalSystematic and Applied Microbiology
Volume21
Issue number3
DOIs
StatePublished - Aug 1998
Externally publishedYes

Keywords

  • 23S rDNA-targeted oligonucleotide probes
  • Enterococci
  • Hybridization
  • Water hygiene

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