Applicability of a yeast oestrogen screen for the detection of oestrogen-like activities in environmental samples

Klaus Rehmann; Karl Werner Schramm; Antonius A. Kettrup

doi:10.1016/S0045-6535(98)00562-1

Applicability of a yeast oestrogen screen for the detection of oestrogen-like activities in environmental samples

Klaus Rehmann, Karl Werner Schramm, Antonius A. Kettrup

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

A (xeno)oestrogen bioassay was introduced, using a genetically modified yeast strain which produces a fusion protein encompassing the human oestrogen receptor hormone binding domain and the yeast GAL4-DNA binding domain. Upon binding of appropriate substances this fusion protein recognises the respective DNA sequence thereby enhancing the transcription of a β-galactosidase reporter gene. The bioassay procedure was evaluated by screening 30 compounds, including some known or suspected (xeno)oestrogens and determining EC₅₀-values for 17β-oestradiol, 1.5 nM, 4-tert.-octylphenol, 6.7 μM and bisphenol A, 104 μM. Toluene extracts from different environmental matrices were tested for their oestrogenic activity. The positive test results obtained with a sewage sludge extract indicated the applicability of this bioassay for environmental monitoring.

Original language	English
Pages (from-to)	3303-3312
Number of pages	10
Journal	Chemosphere
Volume	38
Issue number	14
DOIs	https://doi.org/10.1016/S0045-6535(98)00562-1
State	Published - Jun 1999
Externally published	Yes

Keywords

Bioassay
Endocrine disrupter
Environmental monitoring
Oestrogen
Reporter gene
Sewage sludge
Yeast

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10.1016/S0045-6535(98)00562-1

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abstract = "A (xeno)oestrogen bioassay was introduced, using a genetically modified yeast strain which produces a fusion protein encompassing the human oestrogen receptor hormone binding domain and the yeast GAL4-DNA binding domain. Upon binding of appropriate substances this fusion protein recognises the respective DNA sequence thereby enhancing the transcription of a β-galactosidase reporter gene. The bioassay procedure was evaluated by screening 30 compounds, including some known or suspected (xeno)oestrogens and determining EC50-values for 17β-oestradiol, 1.5 nM, 4-tert.-octylphenol, 6.7 μM and bisphenol A, 104 μM. Toluene extracts from different environmental matrices were tested for their oestrogenic activity. The positive test results obtained with a sewage sludge extract indicated the applicability of this bioassay for environmental monitoring.",

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Applicability of a yeast oestrogen screen for the detection of oestrogen-like activities in environmental samples

Abstract

Keywords

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