Antigenic Sites in Carcinoembryonic Antigen

Sten Hammarstrom; John E. Shively; Raymond J. Paxton; Barbara G. Beatty; Ake Larsson; Rahul Ghosh; Ole Bormer; Franz Buchegger; Jean Pierre Mach; Pierre Burtin; P. Seguin; Bruno Darbouret; F. Degorce; J. Sertour; J. P. Jolu; Abraham Fuks; Holger Kalthoff; Wolff Schmieget; Rudiger Arndt; Gunter Kloppel; Sabine von Kleist; Fritz Grunert; Klaus Schwarz; Yuji Matsuoka; Masahide Kuroki; Christoph Wagener; Teddy Weber; Akira Yachi; Kohzoh Imai; Noriyuki Hishikawa; Masayuki Tsujisaki

Antigenic Sites in Carcinoembryonic Antigen

Sten Hammarstrom, John E. Shively, Raymond J. Paxton, Barbara G. Beatty, Ake Larsson, Rahul Ghosh, Ole Bormer, Franz Buchegger, Jean Pierre Mach, Pierre Burtin, P. Seguin, Bruno Darbouret, F. Degorce, J. Sertour, J. P. Jolu, Abraham Fuks, Holger Kalthoff, Wolff Schmieget, Rudiger Arndt, Gunter KloppelSabine von Kleist, Fritz Grunert, Klaus Schwarz, Yuji Matsuoka, Masahide Kuroki, Christoph Wagener, Teddy Weber, Akira Yachi, Kohzoh Imai, Noriyuki Hishikawa, Masayuki Tsujisaki

Research output: Contribution to journal › Article › peer-review

171 Scopus citations

Abstract

The epitope reactivities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen from 11 different research groups were studied using competitive solid-phase immunoassays. About 60% of all possible combinations of Mabs as inhibitors and as the primary binding antibody were investigated. The inhibition data were analyzed by a specially developed computer program “EPITOPES” which measures concordance and discordance in inhibition patterns between Mabs. The analysis showed that 43 of the 52 Mabs (83%) could be classified into one of five essentially noninteracting epitope groups (GOLD 1–5) containing between four and 15 Mabs each. The epitopes recognized by the Mabs belonging to groups 1 to 5 were peptide in nature. With one or two possible exceptions non-classifiable Mabs were either directed against carbohydrate epitopes (4 Mabs) or were inactive in the tests used. Within epitope groups GOLD 1, 4, and 5 two partially overlapping subgroups were distinguished. Mabs with a high degree of carcinoembryonic antigen specificity generally belonged to epitope groups GOLD 1 and 3.

Original language	English
Pages (from-to)	4852-4858
Number of pages	7
Journal	Cancer Research
Volume	49
Issue number	17
State	Published - 1 Sep 1989
Externally published	Yes

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@article{21901cd35b534b41b37194b945e01d26,

title = "Antigenic Sites in Carcinoembryonic Antigen",

abstract = "The epitope reactivities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen from 11 different research groups were studied using competitive solid-phase immunoassays. About 60\% of all possible combinations of Mabs as inhibitors and as the primary binding antibody were investigated. The inhibition data were analyzed by a specially developed computer program “EPITOPES” which measures concordance and discordance in inhibition patterns between Mabs. The analysis showed that 43 of the 52 Mabs (83\%) could be classified into one of five essentially noninteracting epitope groups (GOLD 1–5) containing between four and 15 Mabs each. The epitopes recognized by the Mabs belonging to groups 1 to 5 were peptide in nature. With one or two possible exceptions non-classifiable Mabs were either directed against carbohydrate epitopes (4 Mabs) or were inactive in the tests used. Within epitope groups GOLD 1, 4, and 5 two partially overlapping subgroups were distinguished. Mabs with a high degree of carcinoembryonic antigen specificity generally belonged to epitope groups GOLD 1 and 3.",

author = "Sten Hammarstrom and Shively, \{John E.\} and Paxton, \{Raymond J.\} and Beatty, \{Barbara G.\} and Ake Larsson and Rahul Ghosh and Ole Bormer and Franz Buchegger and Mach, \{Jean Pierre\} and Pierre Burtin and P. Seguin and Bruno Darbouret and F. Degorce and J. Sertour and Jolu, \{J. P.\} and Abraham Fuks and Holger Kalthoff and Wolff Schmieget and Rudiger Arndt and Gunter Kloppel and \{von Kleist\}, Sabine and Fritz Grunert and Klaus Schwarz and Yuji Matsuoka and Masahide Kuroki and Christoph Wagener and Teddy Weber and Akira Yachi and Kohzoh Imai and Noriyuki Hishikawa and Masayuki Tsujisaki",

year = "1989",

month = sep,

day = "1",

language = "English",

volume = "49",

pages = "4852--4858",

journal = "Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research Inc.",

number = "17",

}

Hammarstrom, S, Shively, JE, Paxton, RJ, Beatty, BG, Larsson, A, Ghosh, R, Bormer, O, Buchegger, F, Mach, JP, Burtin, P, Seguin, P, Darbouret, B, Degorce, F, Sertour, J, Jolu, JP, Fuks, A, Kalthoff, H, Schmieget, W, Arndt, R, Kloppel, G, von Kleist, S, Grunert, F, Schwarz, K, Matsuoka, Y, Kuroki, M, Wagener, C, Weber, T, Yachi, A, Imai, K, Hishikawa, N & Tsujisaki, M 1989, 'Antigenic Sites in Carcinoembryonic Antigen', Cancer Research, vol. 49, no. 17, pp. 4852-4858.

TY - JOUR

T1 - Antigenic Sites in Carcinoembryonic Antigen

AU - Hammarstrom, Sten

AU - Shively, John E.

AU - Paxton, Raymond J.

AU - Beatty, Barbara G.

AU - Larsson, Ake

AU - Ghosh, Rahul

AU - Bormer, Ole

AU - Buchegger, Franz

AU - Mach, Jean Pierre

AU - Burtin, Pierre

AU - Seguin, P.

AU - Darbouret, Bruno

AU - Degorce, F.

AU - Sertour, J.

AU - Jolu, J. P.

AU - Fuks, Abraham

AU - Kalthoff, Holger

AU - Schmieget, Wolff

AU - Arndt, Rudiger

AU - Kloppel, Gunter

AU - von Kleist, Sabine

AU - Grunert, Fritz

AU - Schwarz, Klaus

AU - Matsuoka, Yuji

AU - Kuroki, Masahide

AU - Wagener, Christoph

AU - Weber, Teddy

AU - Yachi, Akira

AU - Imai, Kohzoh

AU - Hishikawa, Noriyuki

AU - Tsujisaki, Masayuki

PY - 1989/9/1

Y1 - 1989/9/1

N2 - The epitope reactivities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen from 11 different research groups were studied using competitive solid-phase immunoassays. About 60% of all possible combinations of Mabs as inhibitors and as the primary binding antibody were investigated. The inhibition data were analyzed by a specially developed computer program “EPITOPES” which measures concordance and discordance in inhibition patterns between Mabs. The analysis showed that 43 of the 52 Mabs (83%) could be classified into one of five essentially noninteracting epitope groups (GOLD 1–5) containing between four and 15 Mabs each. The epitopes recognized by the Mabs belonging to groups 1 to 5 were peptide in nature. With one or two possible exceptions non-classifiable Mabs were either directed against carbohydrate epitopes (4 Mabs) or were inactive in the tests used. Within epitope groups GOLD 1, 4, and 5 two partially overlapping subgroups were distinguished. Mabs with a high degree of carcinoembryonic antigen specificity generally belonged to epitope groups GOLD 1 and 3.

AB - The epitope reactivities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen from 11 different research groups were studied using competitive solid-phase immunoassays. About 60% of all possible combinations of Mabs as inhibitors and as the primary binding antibody were investigated. The inhibition data were analyzed by a specially developed computer program “EPITOPES” which measures concordance and discordance in inhibition patterns between Mabs. The analysis showed that 43 of the 52 Mabs (83%) could be classified into one of five essentially noninteracting epitope groups (GOLD 1–5) containing between four and 15 Mabs each. The epitopes recognized by the Mabs belonging to groups 1 to 5 were peptide in nature. With one or two possible exceptions non-classifiable Mabs were either directed against carbohydrate epitopes (4 Mabs) or were inactive in the tests used. Within epitope groups GOLD 1, 4, and 5 two partially overlapping subgroups were distinguished. Mabs with a high degree of carcinoembryonic antigen specificity generally belonged to epitope groups GOLD 1 and 3.

UR - http://www.scopus.com/inward/record.url?scp=0024407253&partnerID=8YFLogxK

M3 - Article

C2 - 2474375

AN - SCOPUS:0024407253

SN - 0008-5472

VL - 49

SP - 4852

EP - 4858

JO - Cancer Research

JF - Cancer Research

IS - 17

ER -

Antigenic Sites in Carcinoembryonic Antigen

Abstract

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