Antibody incubation of human marrow graft for prevention graft versus host disease

R. J. Haas, G. Janka, B. Netzel, H. Rodt, S. Thierfelder, M. Helmig, R. Eife, B. H. Belohradsky, H. J. Kolb, K. Betke

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

An in vitro incubation of incompatible donor bone marrow by xenogenic anti-T-cell globulin (ATG) suppressed an otherwise lethal GvH reaction in animal models. An application of this principle to clinical bone marrow transplantation was successfully tried in three patients with acute lymphoblastic leukemia. Preparation of the specific anti-human T-cell globulin (ATCG-H) was carried out by absorption of anti-human thymocyte globulin with liver-kidney homogenate, chronic lymphocytic leukemia cells of B-cell type, and erythrocytes. Subsequent testing revealed that the serum still reacted with human T-cells but no longer reduced the number of colony-forming units in culture (CFU-C). All three bone marrow recipients were treated by chemotherapeutic conditioning and total body irradiation followed by grafting of in vitro treated bone marrow from HLA-identical siblings. The transplantation of the bone marrow was well tolerated and no major side effects were encountered. No patient so far (24, 7, 6 months) has shown any signs of GvHD. The in vitro pretransplantation treatment of bone marrow with anti T-globulin may be a new approach to the prevention for GvHD in man.

Original languageEnglish
Pages (from-to)387-397
Number of pages11
JournalBlut
Volume40
Issue number6
DOIs
StatePublished - Jun 1980
Externally publishedYes

Keywords

  • Bone marrow transplantation
  • Preincubation of donor bone marrow
  • Prevention of GvH
  • Specific anti-T-cell globulin

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