Antibacterial efficacy of titanium-containing alloy with silver-nanoparticles enriched diamond-like carbon coatings

Norbert Harrasser, Sebastian Jüssen, Ingo J. Banke, Ralf Kmeth, Ruediger von Eisenhart-Rothe, Bernd Stritzker, Hans Gollwitzer, Rainer Burgkart

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Silver ions (Ag+) have strong bactericidal effects and Ag-coated medical devices proved their effectiveness in reducing infections in revision total joint arthroplasty. We quantitatively determined the antimicrobial potency of different surface treatments on a titanium alloy (Ti), which had been conversed to diamond-like carbon (DLC-Ti) and doped with high (Ag:PVP = 1:2) and low (Ag:PVP = 1:10 and 1:20) concentrations of Ag (Ag-DLC-Ti) with a modified technique of ion implantation. Bacterial adhesion and planktonic growth of clinically relevant bacterial strains (Staphylococcus epidermidis,Staphylococcus aureus, and Pseudomonas aeruginosa) on Ag-DLC-Ti were compared to untreated Ti by quantification of colony forming units on the adherent surface and in the growth medium as well as semiquantitatively by determining the grade of biofilm formation by scanning electron microscopy. (1) A significant (p < 0.05) antimicrobial effect could be found for all Ag-DLC-Ti samples (reduced growth by 5.6–2.5 logarithmic levels). (2) The antimicrobial effect was depending on the tested bacterial strain (most for P. aeruginosa, least for S. aureus). (3) Antimicrobial potency was positively correlated with Ag concentrations. (4) Biofilm formation was decreased by Ag-DLC-Ti surfaces. This study revealed potent antibacterial effects of Ag-DLC-Ti. This may serve as a promising novel approach to close the gap in antimicrobial protection of musculoskeletal implants.

Original languageEnglish
Article number77
Pages (from-to)1-11
Number of pages11
JournalAMB Express
Volume5
Issue number1
DOIs
StatePublished - 1 Dec 2015
Externally publishedYes

Keywords

  • Antibacterial coating
  • Diamond-like carbon
  • Implant-associated infections
  • Silver
  • Titanium

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