Analyzing bioactive effects of the minor hop compound xanthohumol C on human breast cancer cells using quantitative proteomics

Simon Roehrer, Verena Stork, Christina Ludwig, Mirjana Minceva, Jürgen Behr

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Minor prenylated hop compounds have been attracting increasing attention due to their promising anticarcinogenic properties. Even after intensive purification from natural raw extracts, allocating certain activities to single compounds or complex interactions of the main compound with remaining impurities in very low concentration is difficult. In this study, dose-dependent antiproliferative and cytotoxic effects of the promising xanthohumol (XN) analogue xanthohumol C (XNC) were evaluated and compared to XN and a XN-enriched hop extract (XF). It was demonstrated that the cell growth inhibition of human breast cancer cell line (MCF-7) significantly increases after being treated with XNC compared to XN and XF. Based on label-free data-dependent acquisition proteomics, physiological influences on the proteome of MCF-7 cells were analyzed. Different modes of action between XNC and XN treated MCF-7 cells could be postulated. XNC causes ER stress and seems to be involved in cell-cell adhesion, whereas XN influences cell cycles and DNA replication as well as type I interferon signaling pathway. The results demonstrate the utility of using quantitative proteomics for bioactivity screenings of minor hop compounds and underscore the importance of isolating highly pure compounds into their distinct forms to analyze their different and possibly synergistic activities and modes of action.

Original languageEnglish
Article numbere0213469
JournalPLoS ONE
Volume14
Issue number3
DOIs
StatePublished - Mar 2019

Fingerprint

Dive into the research topics of 'Analyzing bioactive effects of the minor hop compound xanthohumol C on human breast cancer cells using quantitative proteomics'. Together they form a unique fingerprint.

Cite this