Analysis of phosphorylated terpenes by electrospray ionization liquid chromatography-tandem mass spectrometry

Iris Ney; Elke Jäger; Markus Herderich; Peter Schreier; Wilfried Schwab

doi:10.1002/(SICI)1099-1565(199609)7:5<233::AID-PCA306>3.0.CO;2-H

Analysis of phosphorylated terpenes by electrospray ionization liquid chromatography-tandem mass spectrometry

Iris Ney, Elke Jäger, Markus Herderich, Peter Schreier, Wilfried Schwab

University of Würzburg

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Linalool, nerol, geraniol, citronellol and α-terpineol were phosphorylated and the products purified by adsorption chromatography on XAD-2. High performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using the electrospray ionization technique (ESI) revealed the occurrence of mono-, di-, tri- and tetraphosphate esters of the respective monoterpenes. In extracts obtained by XAD-2 adsorption chromatography followed by anion exchange chromatography from green tea leaves, phosphate and diphosphate esters of linalool and α-terpineol were identified for the first time by ESI LC-MS/MS. The corresponding monoterpene alcohols were analysed by high resolution gas chromatography-mass spectrometry after hydrolysis of the extract using acid phosphatase (EC 3.1.3.2).

Original language	English
Pages (from-to)	233-236
Number of pages	4
Journal	Phytochemical Analysis
Volume	7
Issue number	5
DOIs	https://doi.org/10.1002/(SICI)1099-1565(199609)7:5<233::AID-PCA306>3.0.CO;2-H
State	Published - 1996
Externally published	Yes

Keywords

Atmospheric pressure ionization
Electrospray mass spectrometry
Green tea leaves
Liquid chromatography-tandem mass spectrometry
Phosphorylated terpenes

Access to Document

10.1002/(SICI)1099-1565(199609)7:5<233::AID-PCA306>3.0.CO;2-H

Cite this

@article{978c013873674815b07e390e2019664f,

title = "Analysis of phosphorylated terpenes by electrospray ionization liquid chromatography-tandem mass spectrometry",

abstract = "Linalool, nerol, geraniol, citronellol and α-terpineol were phosphorylated and the products purified by adsorption chromatography on XAD-2. High performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using the electrospray ionization technique (ESI) revealed the occurrence of mono-, di-, tri- and tetraphosphate esters of the respective monoterpenes. In extracts obtained by XAD-2 adsorption chromatography followed by anion exchange chromatography from green tea leaves, phosphate and diphosphate esters of linalool and α-terpineol were identified for the first time by ESI LC-MS/MS. The corresponding monoterpene alcohols were analysed by high resolution gas chromatography-mass spectrometry after hydrolysis of the extract using acid phosphatase (EC 3.1.3.2).",

keywords = "Atmospheric pressure ionization, Electrospray mass spectrometry, Green tea leaves, Liquid chromatography-tandem mass spectrometry, Phosphorylated terpenes",

author = "Iris Ney and Elke J{\"a}ger and Markus Herderich and Peter Schreier and Wilfried Schwab",

year = "1996",

doi = "10.1002/(SICI)1099-1565(199609)7:5<233::AID-PCA306>3.0.CO;2-H",

language = "English",

volume = "7",

pages = "233--236",

journal = "Phytochemical Analysis",

issn = "0958-0344",

publisher = "John Wiley and Sons Ltd",

number = "5",

}

TY - JOUR

T1 - Analysis of phosphorylated terpenes by electrospray ionization liquid chromatography-tandem mass spectrometry

AU - Ney, Iris

AU - Jäger, Elke

AU - Herderich, Markus

AU - Schreier, Peter

AU - Schwab, Wilfried

PY - 1996

Y1 - 1996

N2 - Linalool, nerol, geraniol, citronellol and α-terpineol were phosphorylated and the products purified by adsorption chromatography on XAD-2. High performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using the electrospray ionization technique (ESI) revealed the occurrence of mono-, di-, tri- and tetraphosphate esters of the respective monoterpenes. In extracts obtained by XAD-2 adsorption chromatography followed by anion exchange chromatography from green tea leaves, phosphate and diphosphate esters of linalool and α-terpineol were identified for the first time by ESI LC-MS/MS. The corresponding monoterpene alcohols were analysed by high resolution gas chromatography-mass spectrometry after hydrolysis of the extract using acid phosphatase (EC 3.1.3.2).

AB - Linalool, nerol, geraniol, citronellol and α-terpineol were phosphorylated and the products purified by adsorption chromatography on XAD-2. High performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using the electrospray ionization technique (ESI) revealed the occurrence of mono-, di-, tri- and tetraphosphate esters of the respective monoterpenes. In extracts obtained by XAD-2 adsorption chromatography followed by anion exchange chromatography from green tea leaves, phosphate and diphosphate esters of linalool and α-terpineol were identified for the first time by ESI LC-MS/MS. The corresponding monoterpene alcohols were analysed by high resolution gas chromatography-mass spectrometry after hydrolysis of the extract using acid phosphatase (EC 3.1.3.2).

KW - Atmospheric pressure ionization

KW - Electrospray mass spectrometry

KW - Green tea leaves

KW - Liquid chromatography-tandem mass spectrometry

KW - Phosphorylated terpenes

UR - http://www.scopus.com/inward/record.url?scp=0029833653&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1099-1565(199609)7:5<233::AID-PCA306>3.0.CO;2-H

DO - 10.1002/(SICI)1099-1565(199609)7:5<233::AID-PCA306>3.0.CO;2-H

M3 - Article

AN - SCOPUS:0029833653

SN - 0958-0344

VL - 7

SP - 233

EP - 236

JO - Phytochemical Analysis

JF - Phytochemical Analysis

IS - 5

ER -

Analysis of phosphorylated terpenes by electrospray ionization liquid chromatography-tandem mass spectrometry

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this