Analysis of DNA methylation content and patterns in plants

Andreas Finke, Wilfried Rozhon, Ales Pecinka

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

8 Scopus citations

Abstract

DNA methylation is an epigenetic modification, which contributes to the regulation of gene expression and chromatin organization, and thus plays a role in many aspects of plant life. Here we present three methods for the detection of DNA methylation in plant tissues: high performance liquid chromatography, methylation-sensitive restriction digest followed by quantitative PCR and bisulfite conversion followed by single read sequencing. These methods are complementary and allow analysis of DNA methylation in samples from both model and non-model plant species.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages277-298
Number of pages22
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1694
ISSN (Print)1064-3745

Keywords

  • Bisulfite sequencing
  • DNA methylation
  • High precision liquid chromatography
  • Methylation- sensitive restriction endonucleases
  • Plants

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