Analysis of carcinogenic polycyclic aromatic hydrocarbons in complex environmental mixtures by LC-APPI-MS/MS

Christoph Hutzler; Andreas Luch; Johannes G. Filser

doi:10.1016/j.aca.2011.07.003

Analysis of carcinogenic polycyclic aromatic hydrocarbons in complex environmental mixtures by LC-APPI-MS/MS

Christoph Hutzler
, Andreas Luch
, Johannes G. Filser

Default

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Here we developed a highly sensitive, fast and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the detection and analysis of 16 different polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs that have been identified as carcinogens and classified according to their biological potency. Comparison to standard analysis procedures based on gas chromatography-mass spectrometry (GC-MS) instrumentation demonstrated an improved easiness of sample preparation and sensitivity of detection achieved with the new LC-MS/MS method employing an atmospheric pressure photoionization (APPI) source attached to an API 4000 mass spectrometer (LC-APPI-MS/MS). The favorable outcome could be confirmed by analyzing complex mixtures such as certain Standard Reference Materials (SRMs) obtained from the National Institute of Standards & Technology (NIST), i.e., SRM 1975 and SRM 2975, and several diesel exhaust soots provided by the German automobile industry. Certified concentrations of individual analytes provided by NIST not only could be confirmed, but additional extremely potent carcinogens such as several isomeric hexacyclic dibenzopyrenes (DBPs), 5-methylchrysene (5-MC), and others have been detected in these crude samples in a concentration range down to below 1ngg ^-1 raw material.

Original language	English
Pages (from-to)	218-224
Number of pages	7
Journal	Analytica Chimica Acta
Volume	702
Issue number	2
DOIs	https://doi.org/10.1016/j.aca.2011.07.003
State	Published - 30 Sep 2011

Keywords

Benzo[a]pyrene
Dibenzo[a,l]pyrene
Dibenzopyrenes
Dinitropyrenes
LC-APPI-MS/MS
PAHs

Access to Document

10.1016/j.aca.2011.07.003

Cite this

@article{9fc8c80f7c9841afa2d0e9c9b7997e3f,

title = "Analysis of carcinogenic polycyclic aromatic hydrocarbons in complex environmental mixtures by LC-APPI-MS/MS",

abstract = "Here we developed a highly sensitive, fast and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the detection and analysis of 16 different polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs that have been identified as carcinogens and classified according to their biological potency. Comparison to standard analysis procedures based on gas chromatography-mass spectrometry (GC-MS) instrumentation demonstrated an improved easiness of sample preparation and sensitivity of detection achieved with the new LC-MS/MS method employing an atmospheric pressure photoionization (APPI) source attached to an API 4000 mass spectrometer (LC-APPI-MS/MS). The favorable outcome could be confirmed by analyzing complex mixtures such as certain Standard Reference Materials (SRMs) obtained from the National Institute of Standards \& Technology (NIST), i.e., SRM 1975 and SRM 2975, and several diesel exhaust soots provided by the German automobile industry. Certified concentrations of individual analytes provided by NIST not only could be confirmed, but additional extremely potent carcinogens such as several isomeric hexacyclic dibenzopyrenes (DBPs), 5-methylchrysene (5-MC), and others have been detected in these crude samples in a concentration range down to below 1ngg -1 raw material.",

keywords = "Benzo[a]pyrene, Dibenzo[a,l]pyrene, Dibenzopyrenes, Dinitropyrenes, LC-APPI-MS/MS, PAHs",

author = "Christoph Hutzler and Andreas Luch and Filser, \{Johannes G.\}",

note = "Funding Information: The financial support provided by FAT (Research Association for Automotive Technology, Frankfurt, Germany) is gratefully acknowledged. The authors thank Ms. Brigitte Semder for excellent technical assistance and Dr. E. Schriever (FAT) for critical comments and helpful discussions. ",

year = "2011",

month = sep,

day = "30",

doi = "10.1016/j.aca.2011.07.003",

language = "English",

volume = "702",

pages = "218--224",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Analysis of carcinogenic polycyclic aromatic hydrocarbons in complex environmental mixtures by LC-APPI-MS/MS

AU - Hutzler, Christoph

AU - Luch, Andreas

AU - Filser, Johannes G.

N1 - Funding Information: The financial support provided by FAT (Research Association for Automotive Technology, Frankfurt, Germany) is gratefully acknowledged. The authors thank Ms. Brigitte Semder for excellent technical assistance and Dr. E. Schriever (FAT) for critical comments and helpful discussions.

PY - 2011/9/30

Y1 - 2011/9/30

N2 - Here we developed a highly sensitive, fast and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the detection and analysis of 16 different polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs that have been identified as carcinogens and classified according to their biological potency. Comparison to standard analysis procedures based on gas chromatography-mass spectrometry (GC-MS) instrumentation demonstrated an improved easiness of sample preparation and sensitivity of detection achieved with the new LC-MS/MS method employing an atmospheric pressure photoionization (APPI) source attached to an API 4000 mass spectrometer (LC-APPI-MS/MS). The favorable outcome could be confirmed by analyzing complex mixtures such as certain Standard Reference Materials (SRMs) obtained from the National Institute of Standards & Technology (NIST), i.e., SRM 1975 and SRM 2975, and several diesel exhaust soots provided by the German automobile industry. Certified concentrations of individual analytes provided by NIST not only could be confirmed, but additional extremely potent carcinogens such as several isomeric hexacyclic dibenzopyrenes (DBPs), 5-methylchrysene (5-MC), and others have been detected in these crude samples in a concentration range down to below 1ngg -1 raw material.

AB - Here we developed a highly sensitive, fast and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the detection and analysis of 16 different polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs that have been identified as carcinogens and classified according to their biological potency. Comparison to standard analysis procedures based on gas chromatography-mass spectrometry (GC-MS) instrumentation demonstrated an improved easiness of sample preparation and sensitivity of detection achieved with the new LC-MS/MS method employing an atmospheric pressure photoionization (APPI) source attached to an API 4000 mass spectrometer (LC-APPI-MS/MS). The favorable outcome could be confirmed by analyzing complex mixtures such as certain Standard Reference Materials (SRMs) obtained from the National Institute of Standards & Technology (NIST), i.e., SRM 1975 and SRM 2975, and several diesel exhaust soots provided by the German automobile industry. Certified concentrations of individual analytes provided by NIST not only could be confirmed, but additional extremely potent carcinogens such as several isomeric hexacyclic dibenzopyrenes (DBPs), 5-methylchrysene (5-MC), and others have been detected in these crude samples in a concentration range down to below 1ngg -1 raw material.

KW - Benzo[a]pyrene

KW - Dibenzo[a,l]pyrene

KW - Dibenzopyrenes

KW - Dinitropyrenes

KW - LC-APPI-MS/MS

KW - PAHs

UR - https://www.scopus.com/pages/publications/80051475645

U2 - 10.1016/j.aca.2011.07.003

DO - 10.1016/j.aca.2011.07.003

M3 - Article

C2 - 21839201

AN - SCOPUS:80051475645

SN - 0003-2670

VL - 702

SP - 218

EP - 224

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 2

ER -

Analysis of carcinogenic polycyclic aromatic hydrocarbons in complex environmental mixtures by LC-APPI-MS/MS

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this