Analysis of banded human chromosomes and in situ hybridization patterns by scanning force microscopy

P. Rasch, U. Wiedemann, J. Wienberg, W. M. Heckl

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Scanning force microscopy was used to analyze banded human chromosomes and in situ hybridization patterns of biotinylated DNA probes. In standard human GTG-banded metaphase chromosome preparations (where GTG is G-banding with trypsin-Giemsa), chromosomal morphology and banding patterns were well preserved during the scanning procedure. The smallest identifiable features were in the range of about 100 nm and are similar to the typical structures seen by electron microscopy. In addition, in situ hybridization of human DNA probes of known chromosomal localization was used to map specific hybridization signals. Imaging of the precipitated crystals at the hybridization site clearly demonstrates the superior resolution of scanning force microscopy compared to conventional microscopy.

Original languageEnglish
Pages (from-to)2509-2511
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number6
DOIs
StatePublished - 15 Mar 1993
Externally publishedYes

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