TY - JOUR
T1 - Analysis of Bacteriophage‐M 13‐DNA Replication in an Escherichia coli Mutant Thermosensitive in DNA Polymerase III
AU - Staudenbauer, Walter L.
AU - OLSEN, William L.
AU - Hofschneider, Peter Hans
PY - 1973/1
Y1 - 1973/1
N2 - The various stages of M 13 DNA replication were investigated in an Escherichia coli dnaE mutant thermosensitive in DNA polymerase III after a shift to the restrictive temperature (45°C). The following results were obtained. (1) DNA polymerase III is not needed for the conversion of the infecting single‐stranded phage DNA into the parental replicative form. (2) Semi‐conservative replicative form replication is completely blocked in the absence of a functional DNA polymerase III. (3) Synthesis of phage‐specific DNA continues after a temperature shift late in the infection. However, due to an inherent thermosensitivity of M 13 single‐stranded circular DNA production no free single strands are detected.
AB - The various stages of M 13 DNA replication were investigated in an Escherichia coli dnaE mutant thermosensitive in DNA polymerase III after a shift to the restrictive temperature (45°C). The following results were obtained. (1) DNA polymerase III is not needed for the conversion of the infecting single‐stranded phage DNA into the parental replicative form. (2) Semi‐conservative replicative form replication is completely blocked in the absence of a functional DNA polymerase III. (3) Synthesis of phage‐specific DNA continues after a temperature shift late in the infection. However, due to an inherent thermosensitivity of M 13 single‐stranded circular DNA production no free single strands are detected.
UR - http://www.scopus.com/inward/record.url?scp=0015923550&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1973.tb02604.x
DO - 10.1111/j.1432-1033.1973.tb02604.x
M3 - Article
C2 - 4569072
AN - SCOPUS:0015923550
SN - 0014-2956
VL - 32
SP - 247
EP - 253
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 2
ER -