Analyses of HBV cccDNA quantification and modification

Yuchen Xia; Daniela Stadler; Chunkyu Ko; Ulrike Protzer

doi:10.1007/978-1-4939-6700-1_6

Analyses of HBV cccDNA quantification and modification

Yuchen Xia
, Daniela Stadler
, Chunkyu Ko
, Ulrike Protzer

Chair of Virology

Technical University of Munich

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

34 Scopus citations

Abstract

Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	59-72
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-4939-6700-1_6
State	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1540
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Covalently closed circular DNA
Deamination
Differential DNA denaturation PCR
Quantitative PCR
Quantitative differential DNA denaturation PCR

Access to Document

10.1007/978-1-4939-6700-1_6

Cite this

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title = "Analyses of HBV cccDNA quantification and modification",

abstract = "Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.",

keywords = "Covalently closed circular DNA, Deamination, Differential DNA denaturation PCR, Quantitative PCR, Quantitative differential DNA denaturation PCR",

author = "Yuchen Xia and Daniela Stadler and Chunkyu Ko and Ulrike Protzer",

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TY - CHAP

T1 - Analyses of HBV cccDNA quantification and modification

AU - Xia, Yuchen

AU - Stadler, Daniela

AU - Ko, Chunkyu

AU - Protzer, Ulrike

N1 - Publisher Copyright: © Springer Science+Business Media LLC 2017.

PY - 2017

Y1 - 2017

N2 - Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.

AB - Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.

KW - Covalently closed circular DNA

KW - Deamination

KW - Differential DNA denaturation PCR

KW - Quantitative PCR

KW - Quantitative differential DNA denaturation PCR

UR - https://www.scopus.com/pages/publications/85006459327

U2 - 10.1007/978-1-4939-6700-1_6

DO - 10.1007/978-1-4939-6700-1_6

M3 - Chapter

C2 - 27975308

AN - SCOPUS:85006459327

T3 - Methods in Molecular Biology

SP - 59

EP - 72

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Analyses of HBV cccDNA quantification and modification

Abstract

Publication series

UN SDGs

Keywords

Access to Document

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