An ISFET-algal (Chlamydomonas) hybrid provides a system for eco- toxicological tests

Dietmar Schubnell; Mirko Lehmann; Werner Baumann; Franz G. Rott; Bernhard Wolf; Christoph F. Beck

doi:10.1016/S0956-5663(99)00025-1

An ISFET-algal (Chlamydomonas) hybrid provides a system for eco- toxicological tests

Dietmar Schubnell, Mirko Lehmann, Werner Baumann, Franz G. Rott, Bernhard Wolf, Christoph F. Beck

University of Freiburg

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A cellular sensoring system was designed in which metabolism-dedicated pH-ISFETs and the unicellular green alga Chlamydomonas reinhardtii as a biological component, were combined. The system permits on-line detection of pH changes caused by the metabolic and photosynthetic activities of the cells. Photosynthetic activity results in a basification of the medium caused by uptake of CO₂. In darkness, an acidification of the medium, resulting from the production of CO₂ by degradation of starch was observed. Both, acidification and basification, are sensitive indicators for the physiological activity of the alga. Experiments using inhibitors of energy metabolism or photosynthesis illustrate the utility of this system for an on- line monitoring of substances of eco-toxicological importance.

Original language	English
Pages (from-to)	465-472
Number of pages	8
Journal	Biosensors and Bioelectronics
Volume	14
Issue number	5
DOIs	https://doi.org/10.1016/S0956-5663(99)00025-1
State	Published - 31 May 1999
Externally published	Yes

Keywords

Cellular pH-sensors
Chlamydomonas
Herbicides
ISFETs
Photosynthesis
Respiration
Unicellular green alga

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10.1016/S0956-5663(99)00025-1

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title = "An ISFET-algal (Chlamydomonas) hybrid provides a system for eco- toxicological tests",

abstract = "A cellular sensoring system was designed in which metabolism-dedicated pH-ISFETs and the unicellular green alga Chlamydomonas reinhardtii as a biological component, were combined. The system permits on-line detection of pH changes caused by the metabolic and photosynthetic activities of the cells. Photosynthetic activity results in a basification of the medium caused by uptake of CO2. In darkness, an acidification of the medium, resulting from the production of CO2 by degradation of starch was observed. Both, acidification and basification, are sensitive indicators for the physiological activity of the alga. Experiments using inhibitors of energy metabolism or photosynthesis illustrate the utility of this system for an on- line monitoring of substances of eco-toxicological importance.",

keywords = "Cellular pH-sensors, Chlamydomonas, Herbicides, ISFETs, Photosynthesis, Respiration, Unicellular green alga",

author = "Dietmar Schubnell and Mirko Lehmann and Werner Baumann and Rott, {Franz G.} and Bernhard Wolf and Beck, {Christoph F.}",

note = "Funding Information: We thank Anja Krieger, Martin Brischwein and Edgar Wagner for helpful suggestions and their willingness to share equipment. We also thank Micronas Intermetall GmbH, Freiburg, for the manufacturing and bonding of the ISFETs. This investigation was supported by grants from the University of Freiburg and the State of Baden-Wuerttemberg (Zukunftsoffensive Junge Generation).",

year = "1999",

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doi = "10.1016/S0956-5663(99)00025-1",

language = "English",

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pages = "465--472",

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T1 - An ISFET-algal (Chlamydomonas) hybrid provides a system for eco- toxicological tests

AU - Schubnell, Dietmar

AU - Lehmann, Mirko

AU - Baumann, Werner

AU - Rott, Franz G.

AU - Wolf, Bernhard

AU - Beck, Christoph F.

N1 - Funding Information: We thank Anja Krieger, Martin Brischwein and Edgar Wagner for helpful suggestions and their willingness to share equipment. We also thank Micronas Intermetall GmbH, Freiburg, for the manufacturing and bonding of the ISFETs. This investigation was supported by grants from the University of Freiburg and the State of Baden-Wuerttemberg (Zukunftsoffensive Junge Generation).

PY - 1999/5/31

Y1 - 1999/5/31

N2 - A cellular sensoring system was designed in which metabolism-dedicated pH-ISFETs and the unicellular green alga Chlamydomonas reinhardtii as a biological component, were combined. The system permits on-line detection of pH changes caused by the metabolic and photosynthetic activities of the cells. Photosynthetic activity results in a basification of the medium caused by uptake of CO2. In darkness, an acidification of the medium, resulting from the production of CO2 by degradation of starch was observed. Both, acidification and basification, are sensitive indicators for the physiological activity of the alga. Experiments using inhibitors of energy metabolism or photosynthesis illustrate the utility of this system for an on- line monitoring of substances of eco-toxicological importance.

AB - A cellular sensoring system was designed in which metabolism-dedicated pH-ISFETs and the unicellular green alga Chlamydomonas reinhardtii as a biological component, were combined. The system permits on-line detection of pH changes caused by the metabolic and photosynthetic activities of the cells. Photosynthetic activity results in a basification of the medium caused by uptake of CO2. In darkness, an acidification of the medium, resulting from the production of CO2 by degradation of starch was observed. Both, acidification and basification, are sensitive indicators for the physiological activity of the alga. Experiments using inhibitors of energy metabolism or photosynthesis illustrate the utility of this system for an on- line monitoring of substances of eco-toxicological importance.

KW - Cellular pH-sensors

KW - Chlamydomonas

KW - Herbicides

KW - ISFETs

KW - Photosynthesis

KW - Respiration

KW - Unicellular green alga

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U2 - 10.1016/S0956-5663(99)00025-1

DO - 10.1016/S0956-5663(99)00025-1

M3 - Article

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AN - SCOPUS:0033620586

SN - 0956-5663

VL - 14

SP - 465

EP - 472

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 5

ER -

An ISFET-algal (Chlamydomonas) hybrid provides a system for eco- toxicological tests

Abstract

Keywords

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