An ISFET-algal (Chlamydomonas) hybrid provides a system for eco- toxicological tests

Dietmar Schubnell, Mirko Lehmann, Werner Baumann, Franz G. Rott, Bernhard Wolf, Christoph F. Beck

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


A cellular sensoring system was designed in which metabolism-dedicated pH-ISFETs and the unicellular green alga Chlamydomonas reinhardtii as a biological component, were combined. The system permits on-line detection of pH changes caused by the metabolic and photosynthetic activities of the cells. Photosynthetic activity results in a basification of the medium caused by uptake of CO2. In darkness, an acidification of the medium, resulting from the production of CO2 by degradation of starch was observed. Both, acidification and basification, are sensitive indicators for the physiological activity of the alga. Experiments using inhibitors of energy metabolism or photosynthesis illustrate the utility of this system for an on- line monitoring of substances of eco-toxicological importance.

Original languageEnglish
Pages (from-to)465-472
Number of pages8
JournalBiosensors and Bioelectronics
Issue number5
StatePublished - 31 May 1999
Externally publishedYes


  • Cellular pH-sensors
  • Chlamydomonas
  • Herbicides
  • ISFETs
  • Photosynthesis
  • Respiration
  • Unicellular green alga


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