TY - JOUR
T1 - An In Vitro model of a parallel-plate perfusion system to study bacterial adherence to graft tissues
AU - Ditkowski, Bartosz
AU - Veloso, Tiago R.
AU - Bezulska-Ditkowska, Martyna
AU - Lubig, Andreas
AU - Jockenhoevel, Stefan
AU - Mela, Petra
AU - Jashari, Ramadan
AU - Gewillig, Marc
AU - Meyns, Bart
AU - Hoylaerts, Marc F.
AU - Heying, Ruth
N1 - Publisher Copyright:
© 2019 Journal of Visualized Experiments.
PY - 2019/1
Y1 - 2019/1
N2 - Various valved conduits and stent-mounted valves are used for right ventricular outflow tract (RVOT) valve replacement in patients with congenital heart disease. When using prosthetic materials however, these grafts are susceptible to bacterial infections and various host responses. Identification of bacterial and host factors that play a vital role in endovascular adherence of microorganisms is of importance to better understand the pathophysiology of the onset of infections such as infective endocarditis (IE) and to develop preventive strategies. Therefore, the development of competent models to investigate bacterial adhesion under physiological shear conditions is necessary. Here, we describe the use of a newly designed in vitro perfusion chamber based on parallel plates that allows the study of bacterial adherence to different components of graft tissues such as exposed extracellular matrix, endothelial cells and inert areas. This method combined with colony-forming unit (CFU) counting is adequate to evaluate the propensity of graft materials towards bacterial adhesion under flow. Further on, the flow chamber system might be used to investigate the role of blood components in bacterial adhesion under shear conditions. We demonstrated that the source of tissue, their surface morphology and bacterial species specificity are not the major determining factors in bacterial adherence to graft tissues by using our in-house designed in vitro perfusion model.
AB - Various valved conduits and stent-mounted valves are used for right ventricular outflow tract (RVOT) valve replacement in patients with congenital heart disease. When using prosthetic materials however, these grafts are susceptible to bacterial infections and various host responses. Identification of bacterial and host factors that play a vital role in endovascular adherence of microorganisms is of importance to better understand the pathophysiology of the onset of infections such as infective endocarditis (IE) and to develop preventive strategies. Therefore, the development of competent models to investigate bacterial adhesion under physiological shear conditions is necessary. Here, we describe the use of a newly designed in vitro perfusion chamber based on parallel plates that allows the study of bacterial adherence to different components of graft tissues such as exposed extracellular matrix, endothelial cells and inert areas. This method combined with colony-forming unit (CFU) counting is adequate to evaluate the propensity of graft materials towards bacterial adhesion under flow. Further on, the flow chamber system might be used to investigate the role of blood components in bacterial adhesion under shear conditions. We demonstrated that the source of tissue, their surface morphology and bacterial species specificity are not the major determining factors in bacterial adherence to graft tissues by using our in-house designed in vitro perfusion model.
KW - Adhesion
KW - Developmental biology
KW - Flow chamber
KW - Infective endocarditis
KW - Issue 143
KW - RVOT
KW - Shear stress
KW - Staphylococcus aureus
KW - Subendothelial matrix
KW - Valvular graft tissues
UR - http://www.scopus.com/inward/record.url?scp=85060145330&partnerID=8YFLogxK
U2 - 10.3791/58476
DO - 10.3791/58476
M3 - Article
C2 - 30663688
AN - SCOPUS:85060145330
SN - 1940-087X
VL - 2019
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 143
M1 - e58476
ER -