An in situ hybridization protocol for detection and identification of planktonic bacteria

Frank Oliver Glöckner; Rudolf Amann; Albin Alfreider; Jakob Pernthaler; Roland Psenner; Karlheinz Trebesius; Karl Heinz Schleifer

doi:10.1016/S0723-2020(96)80069-5

An in situ hybridization protocol for detection and identification of planktonic bacteria

Frank Oliver Glöckner
, Rudolf Amann
, Albin Alfreider
, Jakob Pernthaler
, Roland Psenner
, Karlheinz Trebesius
, Karl Heinz Schleifer

TUM Emeriti of Excellence

Technical University of Munich
University of Innsbruck

Research output: Contribution to journal › Article › peer-review

275 Scopus citations

Abstract

The fraction of planktonic bacteria in oligo- and mesotrophic lakes that can be classified by in situ hybridization has been significantly increased with an optimized protocol. By the use of white polycarbonate filters, CY3-monolabeled oligonucleotide probes, and high quality fluorescence filter sets between 29 and 64% of the cells visualized by 4',6-diamidino-2-phenylindole (DAPI) can be detected with a bacterial probe or group-specific probes by epifluorescence microscopy without further digital image enhancement.

Original language	English
Pages (from-to)	403-406
Number of pages	4
Journal	Systematic and Applied Microbiology
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/S0723-2020(96)80069-5
State	Published - Oct 1996

Keywords

CY3
Epifluorescence microscopy
In situ hybridization
Oligonucleotide probe
Planktonic bacteria
vRNA

Access to Document

10.1016/S0723-2020(96)80069-5

Cite this

@article{5c1f4b4a9e5949faa883480df31e10bb,

title = "An in situ hybridization protocol for detection and identification of planktonic bacteria",

abstract = "The fraction of planktonic bacteria in oligo- and mesotrophic lakes that can be classified by in situ hybridization has been significantly increased with an optimized protocol. By the use of white polycarbonate filters, CY3-monolabeled oligonucleotide probes, and high quality fluorescence filter sets between 29 and 64\% of the cells visualized by 4',6-diamidino-2-phenylindole (DAPI) can be detected with a bacterial probe or group-specific probes by epifluorescence microscopy without further digital image enhancement.",

keywords = "CY3, Epifluorescence microscopy, In situ hybridization, Oligonucleotide probe, Planktonic bacteria, vRNA",

author = "Gl{\"o}ckner, \{Frank Oliver\} and Rudolf Amann and Albin Alfreider and Jakob Pernthaler and Roland Psenner and Karlheinz Trebesius and Schleifer, \{Karl Heinz\}",

year = "1996",

month = oct,

doi = "10.1016/S0723-2020(96)80069-5",

language = "English",

volume = "19",

pages = "403--406",

journal = "Systematic and Applied Microbiology",

issn = "0723-2020",

publisher = "Elsevier GmbH",

number = "3",

}

TY - JOUR

T1 - An in situ hybridization protocol for detection and identification of planktonic bacteria

AU - Glöckner, Frank Oliver

AU - Amann, Rudolf

AU - Alfreider, Albin

AU - Pernthaler, Jakob

AU - Psenner, Roland

AU - Trebesius, Karlheinz

AU - Schleifer, Karl Heinz

PY - 1996/10

Y1 - 1996/10

N2 - The fraction of planktonic bacteria in oligo- and mesotrophic lakes that can be classified by in situ hybridization has been significantly increased with an optimized protocol. By the use of white polycarbonate filters, CY3-monolabeled oligonucleotide probes, and high quality fluorescence filter sets between 29 and 64% of the cells visualized by 4',6-diamidino-2-phenylindole (DAPI) can be detected with a bacterial probe or group-specific probes by epifluorescence microscopy without further digital image enhancement.

AB - The fraction of planktonic bacteria in oligo- and mesotrophic lakes that can be classified by in situ hybridization has been significantly increased with an optimized protocol. By the use of white polycarbonate filters, CY3-monolabeled oligonucleotide probes, and high quality fluorescence filter sets between 29 and 64% of the cells visualized by 4',6-diamidino-2-phenylindole (DAPI) can be detected with a bacterial probe or group-specific probes by epifluorescence microscopy without further digital image enhancement.

KW - CY3

KW - Epifluorescence microscopy

KW - In situ hybridization

KW - Oligonucleotide probe

KW - Planktonic bacteria

KW - vRNA

UR - https://www.scopus.com/pages/publications/0029859085

U2 - 10.1016/S0723-2020(96)80069-5

DO - 10.1016/S0723-2020(96)80069-5

M3 - Article

AN - SCOPUS:0029859085

SN - 0723-2020

VL - 19

SP - 403

EP - 406

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 3

ER -

An in situ hybridization protocol for detection and identification of planktonic bacteria

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this