An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Immanuel Andrä; Hanna Ulrich; Susi Dürr; Dominik Soll; Lynette Henkel; Corinne Angerpointner; Julia Ritter; Sabine Przibilla; Herbert Stadler; Manuel Effenberger; Dirk H. Busch; Matthias Schiemann

doi:10.1002/cyto.a.23964

An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Immanuel Andrä, Hanna Ulrich, Susi Dürr, Dominik Soll, Lynette Henkel, Corinne Angerpointner, Julia Ritter, Sabine Przibilla, Herbert Stadler, Manuel Effenberger, Dirk H. Busch, Matthias Schiemann

Chair of Medical Microbiology, Immunology and Hygiene

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.

Original language	English
Pages (from-to)	171-183
Number of pages	13
Journal	Cytometry Part A
Volume	97
Issue number	2
DOIs	https://doi.org/10.1002/cyto.a.23964
State	Published - 1 Feb 2020

Keywords

MAPK
T cells
cell purification
cell sorting
cell stress
flow cytometry cell sorting
p38 activation
traceless affinity cell selection

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10.1002/cyto.a.23964

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abstract = "Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.",

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AU - Andrä, Immanuel

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AU - Soll, Dominik

AU - Henkel, Lynette

AU - Angerpointner, Corinne

AU - Ritter, Julia

AU - Przibilla, Sabine

AU - Stadler, Herbert

AU - Effenberger, Manuel

AU - Busch, Dirk H.

AU - Schiemann, Matthias

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An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

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