An enzyme immunoassay for the determination of methabenzthiazuron

Sabine KreiBig, Bertold Hock, Ronald Stöcker

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A sensitive enzyme immunoassay is described for the determination of the urea herbicide methabenzthiazuron. The assay is carried out with polyclonal antibodies, which were raised in rabbits by immunization with a methabenzthiazuron-BSA conjugate containing five methabenzthiazuron residues per molecule. The ELISA was optimized on microtiter plates with a peroxidase-methabenzthiazuron tracer. The middle of the test (50% B/B) was found at 1.0μg/l. The lower detection limit of methabenzthiazuron is c. 0.05μ g/l. Samples can be measured up to 10μg/l methabenzthiazuron (upper detection limit). The assay does not require concentration or clean-up steps for drinking or ground water samples. Validation experiments showed a good accuracy and precision. Work with monoclonal antibodies is in progress.

Original languageEnglish
Pages (from-to)1729-1739
Number of pages11
JournalAnalytical Letters
Volume24
Issue number10
DOIs
StatePublished - 1 Oct 1991

Keywords

  • methabenzthiazuron - EIA - ELISA - enzyme immunoassay - herbicide - polyclonal antibodies

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