An efficient protocol for NMR-spectroscopy-based structure determination of protein complexes in solution

Bernd Simon, Tobias Madl, Cameron D. Mackereth, Michael Nilges, Michael Sattler

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

(Figure Presented) Putting the pieces together: An efficient, generally applicable approach for structural analysis of protein complexes and multidomain proteins in solution based on NMR spectroscopy is presented. Starting from available high-resolution structures of individual domains or subunits, the overall domain arrangement is calculated from NMR spectroscopy data that can be obtained for high-molecular-weight complexes.

Original languageEnglish
Pages (from-to)1967-1970
Number of pages4
JournalAngewandte Chemie International Edition in English
Volume49
Issue number11
DOIs
StatePublished - 8 Mar 2010

Keywords

  • NMR spectroscopy
  • Paramagnetic relaxation enhancement
  • Protein structures
  • Residual dipolar coupling
  • Structural biology

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