Alterations of flavonoid biosynthesis in young grapevine (Vitis vinifera L.) leaves, flowers, and berries induced by the dioxygenase inhibitor prohexadione-Ca

Iva Puhl, Florian Stadler, Dieter Treutter

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Prohexadione-Ca is a structural mimic of 2-oxoglutarate, and according to this property, it is able to inhibit dioxygenase enzymes, which require 2-oxoglutarate as a cosubstrate. Such enzymes are involved in flavonoid biosynthesis; therefore, prohexadione-Ca treatment leads to alterations in the flavonoid metabolism in grapevine tissues. Because of the fact that phenolic compounds often are responsible for enhanced plant resistance, modification of phenylpropanoid metabolism using elicitation can be considered as a new potential strategy in plant protection. The phenolic compounds were analyzed by high-performance liquid chromatography combined with chemical reaction detection. Tissue treatment induced the accumulation of unusual flavonoids, which were identified as derivatives of pentahydroxyflavanone, eriodictyol, and luteoliflavan. Concentrations of constitutive flavonoids were also affected by the bioregulator treatment. The alterations of the flavonoid profiles are discussed with respect to substrate preferences of relevant enzymes.

Original languageEnglish
Pages (from-to)2498-2504
Number of pages7
JournalJournal of agricultural and food chemistry
Volume56
Issue number7
DOIs
StatePublished - 9 Apr 2008

Keywords

  • Dioxygenase
  • Eriodictyol
  • Flavanone
  • Flavonol
  • Luteoliflavan
  • Pentahydroxyflavanone

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