All you wanted to know about SELEX

Stefanie J. Klug; Michael Famulok

doi:10.1007/BF00996358

All you wanted to know about SELEX

Stefanie J. Klug, Michael Famulok

University of Munich

Research output: Contribution to journal › Article › peer-review

241 Scopus citations

Abstract

In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications of in vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusive in vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.

Original language	English
Pages (from-to)	97-107
Number of pages	11
Journal	Molecular Biology Reports
Volume	20
Issue number	2
DOIs	https://doi.org/10.1007/BF00996358
State	Published - Jun 1994
Externally published	Yes

Keywords

SELEX
aptamers
combinatorial nucleic acid libraries
in vitro selection
ribozymes

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10.1007/BF00996358

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KW - ribozymes

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All you wanted to know about SELEX

Abstract

Keywords

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