Affinity purification of cellulose-binding enzymes of Clostridium stercorarium

Cellulose-affinity chromatography proved to be a fast and efficient purification procedure for exoenzymes of the thermophilic anaerobe Clostridium stercorarium, suitable for the preparation of large amounts of enzymes for technical applications. The cellulose-binding enzymes could be identified as the C. stercorarium; cellulolytic enzymes Avicelase I and Avicelase II characterized previously as endo-1,4-β-glucanase and exo-1,4- β-glucanase. A third protein was identified as xylanase A, the major endo- 1,4-β-xylanase of this organism.