Advantages of fluorescence-mediated tomography: a prelude to molecular interrogations in deep tissues

Vasilis Ntziachristos; Edward Graves; Ralph Weissleder

Advantages of fluorescence-mediated tomography: a prelude to molecular interrogations in deep tissues

Vasilis Ntziachristos
, Edward Graves
, Ralph Weissleder

Harvard Medical School

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We present certain experimental advantages of fluorescence measurements versus intrinsic contrast measurements pertaining to imaging the distribution of fluorochromes in tissue by means of a normalized Born expansion. Based on these observations we derive an appropriate reconstruction algorithm that concurrently uses intrinsic and fluorescence contrast that facilitates fluorescence-mediated molecular tomography in tissues in-vivo. In contrast to the standard Born approximation, the proposed algorithm does not require instrument calibration or absolute photon fluence measurements. Therefore it is ideally suited for experimental tomographic investigations of tissue in the near-infrared region. We have used this algorithm to image and quantify cancer associated cathepsin B expression in cancer animal models.

Original language	English
Title of host publication	Biomedical Topical Meeting, BIOMED 2002
Publisher	Optica Publishing Group (formerly OSA)
Pages	365-367
Number of pages	3
ISBN (Electronic)	1557527024
State	Published - 2002
Externally published	Yes
Event	Biomedical Topical Meeting, BIOMED 2002 - Miami Beach, United States Duration: 7 Apr 2002 → 10 Apr 2002

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Conference

Conference	Biomedical Topical Meeting, BIOMED 2002
Country/Territory	United States
City	Miami Beach
Period	7/04/02 → 10/04/02

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Cite this

@inproceedings{ebc498dc0a2c4cbea861723c58c0fb93,

title = "Advantages of fluorescence-mediated tomography: a prelude to molecular interrogations in deep tissues",

abstract = "We present certain experimental advantages of fluorescence measurements versus intrinsic contrast measurements pertaining to imaging the distribution of fluorochromes in tissue by means of a normalized Born expansion. Based on these observations we derive an appropriate reconstruction algorithm that concurrently uses intrinsic and fluorescence contrast that facilitates fluorescence-mediated molecular tomography in tissues in-vivo. In contrast to the standard Born approximation, the proposed algorithm does not require instrument calibration or absolute photon fluence measurements. Therefore it is ideally suited for experimental tomographic investigations of tissue in the near-infrared region. We have used this algorithm to image and quantify cancer associated cathepsin B expression in cancer animal models.",

author = "Vasilis Ntziachristos and Edward Graves and Ralph Weissleder",

note = "Publisher Copyright: {\textcopyright} 2002 Optical Society of America.; Biomedical Topical Meeting, BIOMED 2002 ; Conference date: 07-04-2002 Through 10-04-2002",

year = "2002",

language = "English",

series = "Optics InfoBase Conference Papers",

publisher = "Optica Publishing Group (formerly OSA)",

pages = "365--367",

booktitle = "Biomedical Topical Meeting, BIOMED 2002",

}

Advantages of fluorescence-mediated tomography: a prelude to molecular interrogations in deep tissues. / Ntziachristos, Vasilis; Graves, Edward; Weissleder, Ralph.
Biomedical Topical Meeting, BIOMED 2002. Optica Publishing Group (formerly OSA), 2002. p. 365-367 (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Advantages of fluorescence-mediated tomography

T2 - Biomedical Topical Meeting, BIOMED 2002

AU - Ntziachristos, Vasilis

AU - Graves, Edward

AU - Weissleder, Ralph

PY - 2002

Y1 - 2002

N2 - We present certain experimental advantages of fluorescence measurements versus intrinsic contrast measurements pertaining to imaging the distribution of fluorochromes in tissue by means of a normalized Born expansion. Based on these observations we derive an appropriate reconstruction algorithm that concurrently uses intrinsic and fluorescence contrast that facilitates fluorescence-mediated molecular tomography in tissues in-vivo. In contrast to the standard Born approximation, the proposed algorithm does not require instrument calibration or absolute photon fluence measurements. Therefore it is ideally suited for experimental tomographic investigations of tissue in the near-infrared region. We have used this algorithm to image and quantify cancer associated cathepsin B expression in cancer animal models.

AB - We present certain experimental advantages of fluorescence measurements versus intrinsic contrast measurements pertaining to imaging the distribution of fluorochromes in tissue by means of a normalized Born expansion. Based on these observations we derive an appropriate reconstruction algorithm that concurrently uses intrinsic and fluorescence contrast that facilitates fluorescence-mediated molecular tomography in tissues in-vivo. In contrast to the standard Born approximation, the proposed algorithm does not require instrument calibration or absolute photon fluence measurements. Therefore it is ideally suited for experimental tomographic investigations of tissue in the near-infrared region. We have used this algorithm to image and quantify cancer associated cathepsin B expression in cancer animal models.

UR - https://www.scopus.com/pages/publications/85135932637

M3 - Conference contribution

AN - SCOPUS:85135932637

T3 - Optics InfoBase Conference Papers

SP - 365

EP - 367

BT - Biomedical Topical Meeting, BIOMED 2002

PB - Optica Publishing Group (formerly OSA)

Y2 - 7 April 2002 through 10 April 2002

ER -

Advantages of fluorescence-mediated tomography: a prelude to molecular interrogations in deep tissues

Abstract

Publication series

Conference

UN SDGs

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