TY - GEN
T1 - Advantages of fluorescence-mediated tomography
T2 - Biomedical Topical Meeting, BIOMED 2002
AU - Ntziachristos, Vasilis
AU - Graves, Edward
AU - Weissleder, Ralph
N1 - Publisher Copyright:
© 2002 Optical Society of America.
PY - 2002
Y1 - 2002
N2 - We present certain experimental advantages of fluorescence measurements versus intrinsic contrast measurements pertaining to imaging the distribution of fluorochromes in tissue by means of a normalized Born expansion. Based on these observations we derive an appropriate reconstruction algorithm that concurrently uses intrinsic and fluorescence contrast that facilitates fluorescence-mediated molecular tomography in tissues in-vivo. In contrast to the standard Born approximation, the proposed algorithm does not require instrument calibration or absolute photon fluence measurements. Therefore it is ideally suited for experimental tomographic investigations of tissue in the near-infrared region. We have used this algorithm to image and quantify cancer associated cathepsin B expression in cancer animal models.
AB - We present certain experimental advantages of fluorescence measurements versus intrinsic contrast measurements pertaining to imaging the distribution of fluorochromes in tissue by means of a normalized Born expansion. Based on these observations we derive an appropriate reconstruction algorithm that concurrently uses intrinsic and fluorescence contrast that facilitates fluorescence-mediated molecular tomography in tissues in-vivo. In contrast to the standard Born approximation, the proposed algorithm does not require instrument calibration or absolute photon fluence measurements. Therefore it is ideally suited for experimental tomographic investigations of tissue in the near-infrared region. We have used this algorithm to image and quantify cancer associated cathepsin B expression in cancer animal models.
UR - http://www.scopus.com/inward/record.url?scp=85135932637&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85135932637
T3 - Optics InfoBase Conference Papers
SP - 365
EP - 367
BT - Biomedical Topical Meeting, BIOMED 2002
PB - Optica Publishing Group (formerly OSA)
Y2 - 7 April 2002 through 10 April 2002
ER -
