Advantages of fluorescence-mediated tomography: a prelude to molecular interrogations in deep tissues

Vasilis Ntziachristos, Edward Graves, Ralph Weissleder

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


We present certain experimental advantages of fluorescence measurements versus intrinsic contrast measurements pertaining to imaging the distribution of fluorochromes in tissue by means of a normalized Born expansion. Based on these observations we derive an appropriate reconstruction algorithm that concurrently uses intrinsic and fluorescence contrast that facilitates fluorescence-mediated molecular tomography in tissues in-vivo. In contrast to the standard Born approximation, the proposed algorithm does not require instrument calibration or absolute photon fluence measurements. Therefore it is ideally suited for experimental tomographic investigations of tissue in the near-infrared region. We have used this algorithm to image and quantify cancer associated cathepsin B expression in cancer animal models.

Original languageEnglish
Title of host publicationBiomedical Topical Meeting, BIOMED 2002
PublisherOptica Publishing Group (formerly OSA)
Number of pages3
ISBN (Electronic)1557527024
StatePublished - 2002
Externally publishedYes
EventBiomedical Topical Meeting, BIOMED 2002 - Miami Beach, United States
Duration: 7 Apr 200210 Apr 2002

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701


ConferenceBiomedical Topical Meeting, BIOMED 2002
Country/TerritoryUnited States
CityMiami Beach


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