Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes

George Themelis; Athanasios Sarantopoulos; Niels J. Harlaar; Gooitzen Van Dam; Vasilis Ntziachristos

doi:10.1109/ISBI.2010.5490160

Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes

George Themelis, Athanasios Sarantopoulos, Niels J. Harlaar, Gooitzen Van Dam, Vasilis Ntziachristos

Chair of Biological Imaging

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

A new concept aiming to improve surgical outcome by providing real-time surgical feedback on tumor margin delineation is presented. We are using a novel multispectral imaging system for real time measurement of fluorescence probes with molecular specificity to tumor biomarkers. Mice bearing xenograft tumors were used to simulate surgical operations of tumor excision guided by real time fluorescence imaging. Bioluminescence imaging and histopathology were used as gold standards to confirm the in-vivo findings. Results demonstrate the capability of the method to identify tumor negative margins with high specificity and provide real time feedback to the surgeon.

Original language	English
Title of host publication	2010 7th IEEE International Symposium on Biomedical Imaging
Subtitle of host publication	From Nano to Macro, ISBI 2010 - Proceedings
Pages	1010-1012
Number of pages	3
DOIs	https://doi.org/10.1109/ISBI.2010.5490160
State	Published - 2010
Event	7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Rotterdam, Netherlands Duration: 14 Apr 2010 → 17 Apr 2010

Publication series

Name	2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings

Conference

Conference	7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010
Country/Territory	Netherlands
City	Rotterdam
Period	14/04/10 → 17/04/10

Keywords

Imaging fluorescent molecular probe
Intraoperative imaging
Multispectral
Tumor

Access to Document

10.1109/ISBI.2010.5490160

Cite this

Themelis, G., Sarantopoulos, A., Harlaar, N. J., Van Dam, G., & Ntziachristos, V. (2010). Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes. In 2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings (pp. 1010-1012). Article 5490160 (2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings). https://doi.org/10.1109/ISBI.2010.5490160

Themelis, George ; Sarantopoulos, Athanasios ; Harlaar, Niels J. et al. / Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes. 2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings. 2010. pp. 1010-1012 (2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings).

@inproceedings{04e8241a91a746a08414774b5b349bed,

title = "Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes",

abstract = "A new concept aiming to improve surgical outcome by providing real-time surgical feedback on tumor margin delineation is presented. We are using a novel multispectral imaging system for real time measurement of fluorescence probes with molecular specificity to tumor biomarkers. Mice bearing xenograft tumors were used to simulate surgical operations of tumor excision guided by real time fluorescence imaging. Bioluminescence imaging and histopathology were used as gold standards to confirm the in-vivo findings. Results demonstrate the capability of the method to identify tumor negative margins with high specificity and provide real time feedback to the surgeon.",

keywords = "Imaging fluorescent molecular probe, Intraoperative imaging, Multispectral, Tumor",

author = "George Themelis and Athanasios Sarantopoulos and Harlaar, {Niels J.} and {Van Dam}, Gooitzen and Vasilis Ntziachristos",

year = "2010",

doi = "10.1109/ISBI.2010.5490160",

language = "English",

isbn = "9781424441266",

series = "2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings",

pages = "1010--1012",

booktitle = "2010 7th IEEE International Symposium on Biomedical Imaging",

note = "7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 ; Conference date: 14-04-2010 Through 17-04-2010",

}

Themelis, G, Sarantopoulos, A, Harlaar, NJ, Van Dam, G & Ntziachristos, V 2010, Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes. in 2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings., 5490160, 2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings, pp. 1010-1012, 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010, Rotterdam, Netherlands, 14/04/10. https://doi.org/10.1109/ISBI.2010.5490160

Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes. / Themelis, George; Sarantopoulos, Athanasios; Harlaar, Niels J. et al.
2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings. 2010. p. 1010-1012 5490160 (2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes

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AU - Ntziachristos, Vasilis

PY - 2010

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AB - A new concept aiming to improve surgical outcome by providing real-time surgical feedback on tumor margin delineation is presented. We are using a novel multispectral imaging system for real time measurement of fluorescence probes with molecular specificity to tumor biomarkers. Mice bearing xenograft tumors were used to simulate surgical operations of tumor excision guided by real time fluorescence imaging. Bioluminescence imaging and histopathology were used as gold standards to confirm the in-vivo findings. Results demonstrate the capability of the method to identify tumor negative margins with high specificity and provide real time feedback to the surgeon.

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KW - Intraoperative imaging

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KW - Tumor

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Themelis G, Sarantopoulos A, Harlaar NJ, Van Dam G, Ntziachristos V. Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes. In 2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings. 2010. p. 1010-1012. 5490160. (2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2010 - Proceedings). doi: 10.1109/ISBI.2010.5490160

Advancing the technology and applications of surgical fluorescence imaging with targeted fluorochromes

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Keywords

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