Advanced Non-Destructive Ocular Visualization Methods by Improved X-Ray Imaging Techniques

Christian Enders, Eva Maria Braig, Kai Scherer, Jens U. Werner, Gerhard K. Lang, Gabriele E. Lang, Franz Pfeiffer, Peter Noël, Ernst Rummeny, Julia Herzen

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Due to limited X-ray contrast, the use of micro-CT in histology is so far not as widespread as predicted. While specific staining procedures-mostly using iodine-address this shortcoming, long diffusion times restrict its use in the often time-constrained daily routine. Recently, a novel staining protocol has been proposed using a biochemical preconditioning step, which increases the permeability of the cells for the staining agent. This could enable the imaging of entire organs of small mammals at a yet unmatched image quality with reasonable preparation and scan times. We here propose an adaptation of this technique for virtual ophthalmology and histology by volumetrically assessing both human and porcine eyes. Hereby, we demonstrate that (contrast-enhanced) micro-CT can outperform conventional histology in the assessment of tumor entities, as well as functioning as a supplementary tool for surgeons in the positioning of intraocular implants in-vitro and as a general assessment tool for ophthalmologic specimens.

Original languageEnglish
Article numbere0170633
JournalPLoS ONE
Issue number1
StatePublished - Jan 2017


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