TY - JOUR
T1 - Adipose-derived mesenchymal stem cells from liposuction and resected fat are feasible sources for regenerative medicine
AU - Schneider, Sandra
AU - Unger, Marina
AU - Van Griensven, Martijn
AU - Balmayor, Elizabeth R.
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/5/19
Y1 - 2017/5/19
N2 - Background: The use of mesenchymal stem cells (MSCs) in research and in regenerative medicine has progressed. Bone marrow as a source has drawbacks because of subsequent morbidities. An easily accessible and valuable source is adipose tissue. This type of tissue contains a high number of MSCs, and obtaining higher quantities of tissue is more feasible. Fat tissue can be harvested using different methods such as liposuction and resection. First, a detailed isolation protocol with complete characterization is described. This also includes highlighting problems and pitfalls. Furthermore, some comparisons of these different harvesting methods exist. However, the later characterization of the cells is conducted poorly in most cases. Methods: We performed an in-depth characterization over five passages including an investigation of the effect of freezing and thawing. Characterization was performed using flow cytometry with CD markers, metabolic activity with Alamar Blue, growth potential in between passages, and cytoskeleton staining. Results: Our results show that the cells isolated with distinct isolation methods (solid versus liposuction “liquid”) have the same MSC potential. However, the percentage of cells positive for the markers CD73, CD90, and CD105 is initially quite low. The cells isolated from the liquid fat tissue grow faster at higher passages, and significantly more cells display MSC markers. Conclusion: In summary, we show a simple and efficient method to isolate adipose-derived mesenchymal stem cells from different preparations. Liposuctions and resection can be used, whereas liposuction has more growth potential at higher passages.
AB - Background: The use of mesenchymal stem cells (MSCs) in research and in regenerative medicine has progressed. Bone marrow as a source has drawbacks because of subsequent morbidities. An easily accessible and valuable source is adipose tissue. This type of tissue contains a high number of MSCs, and obtaining higher quantities of tissue is more feasible. Fat tissue can be harvested using different methods such as liposuction and resection. First, a detailed isolation protocol with complete characterization is described. This also includes highlighting problems and pitfalls. Furthermore, some comparisons of these different harvesting methods exist. However, the later characterization of the cells is conducted poorly in most cases. Methods: We performed an in-depth characterization over five passages including an investigation of the effect of freezing and thawing. Characterization was performed using flow cytometry with CD markers, metabolic activity with Alamar Blue, growth potential in between passages, and cytoskeleton staining. Results: Our results show that the cells isolated with distinct isolation methods (solid versus liposuction “liquid”) have the same MSC potential. However, the percentage of cells positive for the markers CD73, CD90, and CD105 is initially quite low. The cells isolated from the liquid fat tissue grow faster at higher passages, and significantly more cells display MSC markers. Conclusion: In summary, we show a simple and efficient method to isolate adipose-derived mesenchymal stem cells from different preparations. Liposuctions and resection can be used, whereas liposuction has more growth potential at higher passages.
KW - Adipose tissue
KW - Growth potential
KW - Liposuction
KW - Mesenchymal stem cells
KW - Solid fat
KW - Surface characterization
UR - http://www.scopus.com/inward/record.url?scp=85019932294&partnerID=8YFLogxK
U2 - 10.1186/s40001-017-0258-9
DO - 10.1186/s40001-017-0258-9
M3 - Article
C2 - 28526089
AN - SCOPUS:85019932294
SN - 0949-2321
VL - 22
JO - European Journal of Medical Research
JF - European Journal of Medical Research
IS - 1
M1 - 17
ER -