TY - JOUR
T1 - Addressing uncertainties in correlative imaging of exogenous particles with the tissue microanatomy with synchronous imaging strategies
AU - Morrell, Alexander P.
AU - Martin, Richard A.
AU - Roberts, Helen M.
AU - Castillo-Michel, Hiram
AU - Mosselmans, J. Frederick W.
AU - Geraki, Kalotina
AU - Warfield, Adrian T.
AU - Lingor, Paul
AU - Qayyum, Wasif
AU - Graf, Daniel
AU - Febbraio, Maria
AU - Addison, Owen
N1 - Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press.
PY - 2023/6/1
Y1 - 2023/6/1
N2 - Exposure to exogenous particles is of increasing concern to human health. Characterizing the concentrations, chemical species, distribution, and involvement of the stimulus with the tissue microanatomy is essential in understanding the associated biological response. However, no single imaging technique can interrogate all these features at once, which confounds and limits correlative analyses. Developments of synchronous imaging strategies, allowing multiple features to be identified simultaneously, are essential to assess spatial relationships between these key features with greater confidence. Here, we present data to first highlight complications of correlative analysis between the tissue microanatomy and elemental composition associated with imaging serial tissue sections. This is achieved by assessing both the cellular and elemental distributions in three-dimensional space using optical microscopy on serial sections and confocal X-ray fluorescence spectroscopy on bulk samples, respectively. We propose a new imaging strategy using lanthanide-Tagged antibodies with X-ray fluorescence spectroscopy. Using simulations, a series of lanthanide tags were identified as candidate labels for scenarios where tissue sections are imaged. The feasibility and value of the proposed approach are shown where an exposure of Ti was identified concurrently with CD45 positive cells at sub-cellular resolutions. Significant heterogeneity in the distribution of exogenous particles and cells can be present between immediately adjacent serial sections showing a clear need of synchronous imaging methods. The proposed approach enables elemental compositions to be correlated with the tissue microanatomy in a highly multiplexed and non-destructive manner at high spatial resolutions with the opportunity for subsequent guided analysis.
AB - Exposure to exogenous particles is of increasing concern to human health. Characterizing the concentrations, chemical species, distribution, and involvement of the stimulus with the tissue microanatomy is essential in understanding the associated biological response. However, no single imaging technique can interrogate all these features at once, which confounds and limits correlative analyses. Developments of synchronous imaging strategies, allowing multiple features to be identified simultaneously, are essential to assess spatial relationships between these key features with greater confidence. Here, we present data to first highlight complications of correlative analysis between the tissue microanatomy and elemental composition associated with imaging serial tissue sections. This is achieved by assessing both the cellular and elemental distributions in three-dimensional space using optical microscopy on serial sections and confocal X-ray fluorescence spectroscopy on bulk samples, respectively. We propose a new imaging strategy using lanthanide-Tagged antibodies with X-ray fluorescence spectroscopy. Using simulations, a series of lanthanide tags were identified as candidate labels for scenarios where tissue sections are imaged. The feasibility and value of the proposed approach are shown where an exposure of Ti was identified concurrently with CD45 positive cells at sub-cellular resolutions. Significant heterogeneity in the distribution of exogenous particles and cells can be present between immediately adjacent serial sections showing a clear need of synchronous imaging methods. The proposed approach enables elemental compositions to be correlated with the tissue microanatomy in a highly multiplexed and non-destructive manner at high spatial resolutions with the opportunity for subsequent guided analysis.
KW - correlative imaging
KW - endogenous elemental imaging
KW - exogenous metal imaging
KW - lanthanide X-ray fluorescence
KW - metal-labelled antibodies
KW - synchrotron X-ray fluorescence spectroscopy
KW - synchrotron confocal X-ray fluorescence spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85161991590&partnerID=8YFLogxK
U2 - 10.1093/mtomcs/mfad030
DO - 10.1093/mtomcs/mfad030
M3 - Article
C2 - 37193667
AN - SCOPUS:85161991590
SN - 1756-5901
VL - 15
JO - Metallomics
JF - Metallomics
IS - 6
M1 - mfad030
ER -