TY - JOUR
T1 - Adaptive Use of N2‐Dimethyl‐Substituted Pterins by Cultures of Crithidia fasciculata
AU - ZIEGLER, IRMGARD
AU - BÖHME, MANFRED
AU - PFLEIDERER, WOLFGANG
AU - ELSTNER, ERICH F.
PY - 1981/8
Y1 - 1981/8
N2 - The compound 6‐(L‐erythro‐1,2′,3′‐trihydroxypropyl)pterin, at a concentration of 50 pg/ml (“L‐erythro‐neopteria”), supports half‐maximal growth of Crithidia fasciculata; biopterin at a concentration of 30 pg/ml is shown to yield similar growth. N2‐dimethyl‐6‐(L‐erythro‐1′,2′,3′‐trihydroxypropyl)pterin (A) was inactive even at 100 ng/ml. Synergism was observed with the N2‐dimethylamino derivative (A) in the presence of suboptimal biopterin, its activity then being of the order of L‐erythro‐neopterin. In contrast, the stereoisomeric N2‐dimethyl‐6‐(D‐erythro‐1′,2′,3′‐trihydroxypropyl)pterin (“dimethyl‐D‐erythro‐neopterin”) and its 3′‐mono‐phosphate only slightly enhanced growth under similar conditions but both threo‐isomers had no supplementary activity. Biopterin‐induced growth was slowed by 6‐(D‐erythro1′,2′,3′‐trihydroxypropyl)pterin (D‐neopterin); the threo‐isomers had no such effect. An adaptive demethylation capacity by growing cultures and competition of biopterin uptake by D‐neopterin seems likely. The report of the occurrence in Euglena of N2‐dimethyl‐6‐(L‐threo‐1′,2′,3′‐trihydroxypropyl)pterin and its 3′‐mono‐phosphate adds further interest to our observations.
AB - The compound 6‐(L‐erythro‐1,2′,3′‐trihydroxypropyl)pterin, at a concentration of 50 pg/ml (“L‐erythro‐neopteria”), supports half‐maximal growth of Crithidia fasciculata; biopterin at a concentration of 30 pg/ml is shown to yield similar growth. N2‐dimethyl‐6‐(L‐erythro‐1′,2′,3′‐trihydroxypropyl)pterin (A) was inactive even at 100 ng/ml. Synergism was observed with the N2‐dimethylamino derivative (A) in the presence of suboptimal biopterin, its activity then being of the order of L‐erythro‐neopterin. In contrast, the stereoisomeric N2‐dimethyl‐6‐(D‐erythro‐1′,2′,3′‐trihydroxypropyl)pterin (“dimethyl‐D‐erythro‐neopterin”) and its 3′‐mono‐phosphate only slightly enhanced growth under similar conditions but both threo‐isomers had no supplementary activity. Biopterin‐induced growth was slowed by 6‐(D‐erythro1′,2′,3′‐trihydroxypropyl)pterin (D‐neopterin); the threo‐isomers had no such effect. An adaptive demethylation capacity by growing cultures and competition of biopterin uptake by D‐neopterin seems likely. The report of the occurrence in Euglena of N2‐dimethyl‐6‐(L‐threo‐1′,2′,3′‐trihydroxypropyl)pterin and its 3′‐mono‐phosphate adds further interest to our observations.
UR - http://www.scopus.com/inward/record.url?scp=0019790216&partnerID=8YFLogxK
U2 - 10.1111/j.1550-7408.1981.tb02865.x
DO - 10.1111/j.1550-7408.1981.tb02865.x
M3 - Article
AN - SCOPUS:0019790216
SN - 0022-3921
VL - 28
SP - 354
EP - 357
JO - The Journal of Protozoology
JF - The Journal of Protozoology
IS - 3
ER -