TY - JOUR
T1 - Acute stress regulation of neuroplasticity genes in mouse hippocampus CA3 area - Possible novel signalling pathways
AU - Tsolakidou, A.
AU - Trümbach, D.
AU - Panhuysen, M.
AU - Pütz, B.
AU - Deussing, J.
AU - Wurst, W.
AU - Sillaber, I.
AU - Holsboer, F.
AU - Rein, T.
N1 - Funding Information:
We thank Claudia Kühne and Dr Chiara Onofri for technical advice on the microarray and immunochemistry protocols respectively. This work has been funded in part by the Federal Ministry of Education and Research (BMBF) in the framework of the National Genome Research Network (NGFN) Förderkennzeichen 01GR0430.
PY - 2008/7
Y1 - 2008/7
N2 - Stress exposure can lead to the precipitation of psychiatric disorders in susceptible individuals, but the molecular underpinnings are incompletely understood. We used forced swimming in mice to reveal stress-regulated genes in the CA3 area of the hippocampus. To determine changes in the transcriptional profile 4 h and 8 h after stress exposure microarrays were used in the two mouse strains C57BL/6J and DBA/2J, which are known for their differential stress response. We discovered a surprisingly distinct set of regulated genes for each strain and followed selected ones by in situ hybridisation. Our results support the concept of a phased transcriptional reaction to stress. Moreover, we suggest novel stress-elicited pathways, which comprise a number of genes involved in the regulation of neuronal plasticity. Furthermore, we focused in particular on dihydropyrimidinase like 2, to which we provide evidence for its regulation by NeuroD, an important factor for neuronal activity-dependent dendritic morphogenesis.
AB - Stress exposure can lead to the precipitation of psychiatric disorders in susceptible individuals, but the molecular underpinnings are incompletely understood. We used forced swimming in mice to reveal stress-regulated genes in the CA3 area of the hippocampus. To determine changes in the transcriptional profile 4 h and 8 h after stress exposure microarrays were used in the two mouse strains C57BL/6J and DBA/2J, which are known for their differential stress response. We discovered a surprisingly distinct set of regulated genes for each strain and followed selected ones by in situ hybridisation. Our results support the concept of a phased transcriptional reaction to stress. Moreover, we suggest novel stress-elicited pathways, which comprise a number of genes involved in the regulation of neuronal plasticity. Furthermore, we focused in particular on dihydropyrimidinase like 2, to which we provide evidence for its regulation by NeuroD, an important factor for neuronal activity-dependent dendritic morphogenesis.
UR - http://www.scopus.com/inward/record.url?scp=45649085678&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2008.04.005
DO - 10.1016/j.mcn.2008.04.005
M3 - Article
C2 - 18524625
AN - SCOPUS:45649085678
SN - 1044-7431
VL - 38
SP - 444
EP - 452
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 3
ER -