Activity of native hydrolytic enzymes and their association with the cell wall of three ectomycorrhizal fungi

Alfredo Pérez-de-Mora, Bianca Reuter, Marianna Lucio, Alfred Ahne, Michael Schloter, Karin Pritsch

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The ecological and biogeochemical relevance of hydrolytic enzymes associated with the fungal cell wall has been poorly studied in ectomycorrhizal (ECM) fungi. We used a modified sequential extraction procedure to investigate the activity of various hydrolytic enzymes (β-glucosidase, acid-phosphatase, leucine-aminopeptidase, chitinase, xylanase and glucuronidase) and their association with the cell wall of three ECM fungi (Rhizopogon roseolus, Paxillus involutus and Piloderma croceum). Fungi were grown on C-rich solid medium under three different P concentrations (3. 7, 0. 37 and 0. 037 mM). The sequential extraction procedure classifies enzymes as: (a) cytosolic, (b) loosely bound, (c) hydrophobically bound, (d) ionically bound and (e) covalently bound. Results showed that for the same fungus absolute enzymatic activity was affected by P concentration, whilst enzymatic compartmentalization among the cytosol and the cell wall fractions was not. The association of enzymes with the cell wall was fungus- and enzyme-specific. Our data indicate also that enzymes best known for being either extracellular or cytosolic or both, do act in muro as well. The ecological implications of cell wall-bound enzymes and the potential applications and limitations of sequential extractions are further discussed.

Original languageEnglish
Pages (from-to)185-197
Number of pages13
JournalMycorrhiza
Volume23
Issue number3
DOIs
StatePublished - Apr 2013
Externally publishedYes

Keywords

  • Cell wall-bound enzymes
  • General linear model
  • Multivariate analysis
  • Paxillus involutus
  • Piloderma croceum
  • Rhizopogon roseolus
  • Sequential extraction

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