Acoustofluidic separation of biomolecules using carrier microspheres

We have proposed a biomolecule separation technique to selectively isolate targeted proteins from a sample by controlling the motion of microspherical protein-carriers using acoustic waves (see Figure 1). The targeted proteins (1 and 2) inside a heterogeneous sample are conjugated with custom designed functionalized polystyrene microspheres (green and orange). The sample fluid is continuously pumped through an acoustofluidic chip where the targeted proteins (1 and 2) attached to the selected carrier-microspheres are separated from the non-specific protein (3) through different outlet ports of a microfluidic channel. The separated samples are collected, post-processed and run through a polyacrylamide gel electrophoresis (PAGE) for confirmation.