Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine

Holger Heuer; Qodiah Solehati; Ute Zimmerling; Kristina Kleineidam; Michael Schloter; Tanja Müller; Andreas Focks; Sören Thiele-Bruhn; Kornelia Smalla

doi:10.1128/AEM.02577-10

Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine

Holger Heuer, Qodiah Solehati, Ute Zimmerling, Kristina Kleineidam, Michael Schloter, Tanja Müller, Andreas Focks, Sören Thiele-Bruhn, Kornelia Smalla

Research output: Contribution to journal › Article › peer-review

169 Scopus citations

Abstract

Two soils were amended three times with pig manure. The abundance of sulfonamide resistance genes was determined by quantitative PCR 2 months after each application. In both soils treated with sulfadiazinecontaining manure, the numbers of copies of sul1 and sul2 significantly increased compared to numbers after treatments with antibiotic-free manure or a control and accumulated with repeated applications.

Original language	English
Pages (from-to)	2527-2530
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	77
Issue number	7
DOIs	https://doi.org/10.1128/AEM.02577-10
State	Published - Apr 2011
Externally published	Yes

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title = "Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine",

abstract = "Two soils were amended three times with pig manure. The abundance of sulfonamide resistance genes was determined by quantitative PCR 2 months after each application. In both soils treated with sulfadiazinecontaining manure, the numbers of copies of sul1 and sul2 significantly increased compared to numbers after treatments with antibiotic-free manure or a control and accumulated with repeated applications.",

author = "Holger Heuer and Qodiah Solehati and Ute Zimmerling and Kristina Kleineidam and Michael Schloter and Tanja M{\"u}ller and Andreas Focks and S{\"o}ren Thiele-Bruhn and Kornelia Smalla",

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T1 - Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine

AU - Heuer, Holger

AU - Solehati, Qodiah

AU - Zimmerling, Ute

AU - Kleineidam, Kristina

AU - Schloter, Michael

AU - Müller, Tanja

AU - Focks, Andreas

AU - Thiele-Bruhn, Sören

AU - Smalla, Kornelia

PY - 2011/4

Y1 - 2011/4

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AB - Two soils were amended three times with pig manure. The abundance of sulfonamide resistance genes was determined by quantitative PCR 2 months after each application. In both soils treated with sulfadiazinecontaining manure, the numbers of copies of sul1 and sul2 significantly increased compared to numbers after treatments with antibiotic-free manure or a control and accumulated with repeated applications.

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U2 - 10.1128/AEM.02577-10

DO - 10.1128/AEM.02577-10

M3 - Article

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SN - 0099-2240

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JO - Applied and Environmental Microbiology

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Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine

Abstract

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